1cr5
From Proteopedia
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'''N-TERMINAL DOMAIN OF SEC18P''' | '''N-TERMINAL DOMAIN OF SEC18P''' | ||
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[[Category: Babor, S M.]] | [[Category: Babor, S M.]] | ||
[[Category: Fass, D.]] | [[Category: Fass, D.]] | ||
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| - | [[Category: | + | [[Category: Vesicle fusion]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 13:01:55 2008'' | |
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Revision as of 10:01, 2 May 2008
N-TERMINAL DOMAIN OF SEC18P
Overview
Yeast Sec18p and its mammalian orthologue N-ethylmaleimide-sensitive fusion protein (NSF) are hexameric ATPases with a central role in vesicle trafficking. Aided by soluble adapter factors (SNAPs), Sec18p/NSF induces ATP-dependent disassembly of a complex of integral membrane proteins from the vesicle and target membranes (SNAP receptors). During the ATP hydrolysis cycle, the Sec18p/NSF homohexamer undergoes a large-scale conformational change involving repositioning of the most N terminal of the three domains of each protomer, a domain that is required for SNAP-mediated interaction with SNAP receptors. Whether an internal conformational change in the N-terminal domains accompanies their reorientation with respect to the rest of the hexamer remains to be addressed. We have determined the structure of the N-terminal domain from Sec18p by x-ray crystallography. The Sec18p N-terminal domain consists of two beta-sheet-rich subdomains connected by a short linker. A conserved basic cleft opposite the linker may constitute a SNAP-binding site. Despite structural variability in the linker region and in an adjacent loop, all three independent molecules in the crystal asymmetric unit have the identical subdomain interface, supporting the notion that this interface is a preferred packing arrangement. However, the linker flexibility allows for the possibility that other subdomain orientations may be sampled.
About this Structure
1CR5 is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Crystal structure of the Sec18p N-terminal domain., Babor SM, Fass D, Proc Natl Acad Sci U S A. 1999 Dec 21;96(26):14759-64. PMID:10611286 Page seeded by OCA on Fri May 2 13:01:55 2008
