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2ami

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Revision as of 06:44, 9 May 2018

Solution Structure Of The Calcium-loaded N-Terminal Sensor Domain Of Centrin

Structural highlights

2ami is a 1 chain structure with sequence from Chlre. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[CATR_CHLRE] This calcium-binding protein is found in the basal body complexes (the functional homolog of the centrosome in animal cell). In mitotic cells it is specifically associated with the poles of the mitotic spindles at the sites of the duplicated basal body complexes.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Centrin is an essential component of microtubule-organizing centers in organisms ranging from algae and yeast to humans. It is an EF-hand calcium-binding protein with homology to calmodulin but distinct calcium binding properties. In a previously proposed model, the C-terminal domain of centrin serves as a constitutive anchor to target proteins, and the N-terminal domain serves as the sensor of calcium signals. The three-dimensional structure of the N-terminal domain of Chlamydomonas rheinhardtii centrin has been determined in the presence of calcium by solution NMR spectroscopy. The domain is found to occupy an open conformation typical of EF-hand calcium sensors. Comparison of the N- and C-terminal domains of centrin reveals a structural and biochemical basis for the domain specificity of interactions with its cellular targets and the distinct nature of centrin relative to other EF-hand proteins. An NMR titration of the centrin N-terminal domain with a fragment of the known centrin target Sfi1 reveals binding of the peptide to a discrete site on the protein, which supports the proposal that the N-terminal domain serves as a calcium sensor in centrin.

Structure of the N-terminal calcium sensor domain of centrin reveals the biochemical basis for domain-specific function.,Sheehan JH, Bunick CG, Hu H, Fagan PA, Meyn SM, Chazin WJ J Biol Chem. 2006 Feb 3;281(5):2876-81. Epub 2005 Nov 29. PMID:16317001^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Sheehan JH, Bunick CG, Hu H, Fagan PA, Meyn SM, Chazin WJ. Structure of the N-terminal calcium sensor domain of centrin reveals the biochemical basis for domain-specific function. J Biol Chem. 2006 Feb 3;281(5):2876-81. Epub 2005 Nov 29. PMID:16317001 doi:http://dx.doi.org/M509886200

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2ami"

@@ Line 1: / Line 1: @@
 ==Solution Structure Of The Calcium-loaded N-Terminal Sensor Domain Of Centrin==
 <StructureSection load='2ami' size='340' side='right' caption='[[2ami]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
 == Structural highlights ==
 <table><tr><td colspan='2'>[[2ami]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlre Chlre]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AMI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AMI FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ami FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ami OCA], [http://pdbe.org/2ami PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ami RCSB], [http://www.ebi.ac.uk/pdbsum/2ami PDBsum]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ami FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ami OCA], [http://pdbe.org/2ami PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ami RCSB], [http://www.ebi.ac.uk/pdbsum/2ami PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ami ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 11: / Line 12: @@
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/am/2ami_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/am/2ami_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>

2ami

From Proteopedia

Revision as of 06:44, 9 May 2018

Solution Structure Of The Calcium-loaded N-Terminal Sensor Domain Of Centrin

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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