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1mdv

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Revision as of 08:00, 31 January 2018

KEY ROLE OF PHENYLALANINE 20 IN CYTOCHROME C3: STRUCTURE, STABILITY AND FUNCTION STUDIES

Structural highlights

1mdv is a 2 chain structure with sequence from Desvh. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Gene:	CYC (DESVH)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[CYC3_DESVH] Participates in sulfate respiration coupled with phosphorylation by transferring electrons from the enzyme dehydrogenase to ferredoxin.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Aromatic residues in c-type cytochromes might have an important function in the folding and/or electron transferring properties of the molecule. In the tetraheme cytochrome c3 (Mr 13 000) from Desulfovibrio vulgaris Hildenborough, Phe20, is located between heme 1 and heme 3 with its aromatic ring close and almost parallel to the ring plane of heme 1. We replaced this residue by a nonaromatic hydrophobe residue, leucine, and analyzed the effects in terms of functional, structural, and physicochemical properties. While the F20L replacement did not have any strong effects on the heme region stability, a decrease of the thermostability of the whole molecule was observed. In the same way, the four macroscopic redox potentials were affected by the mutation as well as the flexibility of the surface loop around heme 4. The F20L replacement itself and/or this structural modification might be responsible for the loss of the intermolecular cooperativity between F20L cytochrome c3 molecules.

Key role of phenylalanine 20 in cytochrome c3: structure, stability, and function studies.,Dolla A, Arnoux P, Protasevich I, Lobachov V, Brugna M, Giudici-Orticoni MT, Haser R, Czjzek M, Makarov A, Bruschi M Biochemistry. 1999 Jan 5;38(1):33-41. PMID:9890880^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Dolla A, Arnoux P, Protasevich I, Lobachov V, Brugna M, Giudici-Orticoni MT, Haser R, Czjzek M, Makarov A, Bruschi M. Key role of phenylalanine 20 in cytochrome c3: structure, stability, and function studies. Biochemistry. 1999 Jan 5;38(1):33-41. PMID:9890880 doi:10.1021/bi981593h

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1mdv"

@@ Line 1: / Line 1: @@
 ==KEY ROLE OF PHENYLALANINE 20 IN CYTOCHROME C3: STRUCTURE, STABILITY AND FUNCTION STUDIES==
 <StructureSection load='1mdv' size='340' side='right' caption='[[1mdv]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CYC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=882 DESVH])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mdv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mdv OCA], [http://pdbe.org/1mdv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mdv RCSB], [http://www.ebi.ac.uk/pdbsum/1mdv PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mdv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mdv OCA], [http://pdbe.org/1mdv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mdv RCSB], [http://www.ebi.ac.uk/pdbsum/1mdv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1mdv ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 13: / Line 14: @@
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/md/1mdv_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/md/1mdv_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
@@ Line 28: / Line 29: @@
 </div>
 <div class="pdbe-citations 1mdv" style="background-color:#fffaf0;"></div>
-==See Also==
-*[[Cytochrome c|Cytochrome c]]
 == References ==
 <references/>

1mdv

From Proteopedia

Revision as of 08:00, 31 January 2018

KEY ROLE OF PHENYLALANINE 20 IN CYTOCHROME C3: STRUCTURE, STABILITY AND FUNCTION STUDIES

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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