This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

1nwz

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 07:14, 7 February 2018

PYP Ultra-high resolution structure of a Bacterial Photoreceptor

Structural highlights

1nwz is a 1 chain structure with sequence from Dsm 244. The March 2015 RCSB PDB Molecule of the Month feature on Phototropin by David Goodsell is 10.2210/rcsb_pdb/mom_2015_3. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Gene:	PYP (DSM 244)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[PYP_HALHA] Photoactive blue light protein. Probably functions as a photoreceptor for a negative phototaxis response.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Protein photoreceptors use small-molecule cofactors called chromophores to detect light. Only under the influence of the receptors' active sites do these chromophores adopt spectral and photochemical properties that suit the receptors' functional requirements. This protein-induced change in chromophore properties is called photochemical tuning and is a prime example for the general--but poorly understood--process of chemical tuning through which proteins shape the reactivity of their active-site groups. Here we report the 0.82-A resolution X-ray structure of the bacterial light receptor photoactive yellow protein (PYP). The unusually precise structure reveals deviations from expected molecular geometries and anisotropic atomic displacements in the PYP active site. Our analysis of these deviations points directly to the intramolecular forces and active-site dynamics that tune the properties of PYP's chromophore to absorb blue light, suppress fluorescence, and favor the required light-driven double-bond isomerization.

Anticipatory active-site motions and chromophore distortion prime photoreceptor PYP for light activation.,Getzoff ED, Gutwin KN, Genick UK Nat Struct Biol. 2003 Aug;10(8):663-8. PMID:12872160^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Getzoff ED, Gutwin KN, Genick UK. Anticipatory active-site motions and chromophore distortion prime photoreceptor PYP for light activation. Nat Struct Biol. 2003 Aug;10(8):663-8. PMID:12872160 doi:10.1038/nsb958

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1nwz"

@@ Line 1: / Line 1: @@
 ==PYP Ultra-high resolution structure of a Bacterial Photoreceptor==
 <StructureSection load='1nwz' size='340' side='right' caption='[[1nwz]], [[Resolution|resolution]] 0.82&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HC4:4-HYDROXYCINNAMIC+ACID'>HC4</scene></td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PYP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1053 DSM 244])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nwz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nwz OCA], [http://pdbe.org/1nwz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1nwz RCSB], [http://www.ebi.ac.uk/pdbsum/1nwz PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nwz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nwz OCA], [http://pdbe.org/1nwz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1nwz RCSB], [http://www.ebi.ac.uk/pdbsum/1nwz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1nwz ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 13: / Line 14: @@
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nw/1nwz_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nw/1nwz_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>

1nwz

From Proteopedia

Revision as of 07:14, 7 February 2018

PYP Ultra-high resolution structure of a Bacterial Photoreceptor

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox