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1vyi

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Revision as of 07:18, 4 April 2018

Structure of the c-terminal domain of the polymerase cofactor of rabies virus: insights in function and evolution.

Structural highlights

1vyi is a 1 chain structure with sequence from Rabies virus (strain cvs-11). Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Activity:	RNA-directed RNA polymerase, with EC number 2.7.7.48
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[PHOSP_RABVC] Non catalytic polymerase cofactor and regulatory protein that plays a role in viral transcription and replication. Stabilizes the RNA polymerase L to the N-RNA template and binds the soluble protein N, preventing it from encapsidating non-genomic RNA. Also inhibits host IFN-alpha and IFN-beta signaling by binding and retaining phosphorylated STAT1 in the cytoplasm or by inhibiting the DNA binding of STAT1 in the nucleus. Might be involved, through interaction with host dynein, in intracellular microtubule-dependent virus transport of incoming virus from the synapse toward the cell body.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The phosphoprotein (P) of rabies virus binds the viral polymerase to the nucleoprotein (N)-RNA template for transcription and replication. By limited protease digestion we defined a monomeric C-terminal domain of P that can bind to N-RNA. The atomic structure of this domain was determined and previously described mutations that interfere with binding of P to N-RNA could now be interpreted. There appears to be two features involved in this activity situated at opposite surfaces of the molecule: a positively charged patch and a hydrophobic pocket with an exposed tryptophan side-chain. Other previously published work suggests a conformational change in P when it binds to N-RNA, which may imply the repositioning of two helices that would expose a hydrophobic groove for interaction with N. This domain of rabies virus P is structurally unrelated to the N-RNA binding domains of the phosphoproteins of Sendai and measles virus that are members of the same order of viruses, the non-segmented negative strand RNA viruses. The implications of this finding for the evolution of this virus group are discussed.

Structure and function of the C-terminal domain of the polymerase cofactor of rabies virus.,Mavrakis M, McCarthy AA, Roche S, Blondel D, Ruigrok RW J Mol Biol. 2004 Oct 29;343(4):819-31. PMID:15476803^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Mavrakis M, McCarthy AA, Roche S, Blondel D, Ruigrok RW. Structure and function of the C-terminal domain of the polymerase cofactor of rabies virus. J Mol Biol. 2004 Oct 29;343(4):819-31. PMID:15476803 doi:10.1016/j.jmb.2004.08.071

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1vyi"

@@ Line 1: / Line 1: @@
-==STRUCTURE OF THE C-TERMINAL DOMAIN OF THE POLYMERASE COFACTOR OF RABIES VIRUS: INSIGHTS IN FUNCTION AND EVOLUTION.==
+==Structure of the c-terminal domain of the polymerase cofactor of rabies virus: insights in function and evolution.==
 <StructureSection load='1vyi' size='340' side='right' caption='[[1vyi]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1vyi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Rabies_virus_cvs-11 Rabies virus cvs-11]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VYI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1VYI FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1vyi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Rabies_virus_(strain_cvs-11) Rabies virus (strain cvs-11)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VYI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1VYI FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vyi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vyi OCA], [http://pdbe.org/1vyi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1vyi RCSB], [http://www.ebi.ac.uk/pdbsum/1vyi PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vyi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vyi OCA], [http://pdbe.org/1vyi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1vyi RCSB], [http://www.ebi.ac.uk/pdbsum/1vyi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1vyi ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 13: / Line 14: @@
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vy/1vyi_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vy/1vyi_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
@@ Line 36: / Line 37: @@
 </StructureSection>
 [[Category: RNA-directed RNA polymerase]]
-[[Category: Rabies virus cvs-11]]
 [[Category: Blondel, D]]
 [[Category: Mavrakis, M]]
-[[Category: Mccarthy, A A]]
+[[Category: McCarthy, A A]]
 [[Category: Roche, S]]
 [[Category: Ruigrok, R W.H]]

1vyi

From Proteopedia

Revision as of 07:18, 4 April 2018

Structure of the c-terminal domain of the polymerase cofactor of rabies virus: insights in function and evolution.

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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