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2uyz

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Revision as of 06:52, 28 March 2018

Non-covalent complex between Ubc9 and SUMO1

Structural highlights

2uyz is a 2 chain structure with sequence from Human and Lk3 transgenic mice. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	1u9a, 1u9b, 1a5r, 1tgz, 1wyw, 1y8r, 1z5s, 2asq, 2bf8, 2g4d, 2io2, 2iy0, 2iy1
Activity:	Ubiquitin--protein ligase, with EC number 6.3.2.19
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[UBC9_MOUSE] Accepts the ubiquitin-like proteins SUMO1, SUMO2 and SUMO3 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Can catalyze the formation of poly-SUMO chains. Essential for nuclear architecture, chromosome segregation and embryonic viability. Necessary for sumoylation of FOXL2 and KAT5 (By similarity).^[1] ^[2]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The ubiquitin-related modifier SUMO regulates a wide range of cellular processes by post-translational modification with one, or a chain of SUMO molecules. Sumoylation is achieved by the sequential action of several enzymes in which the E2, Ubc9, transfers SUMO from the E1 to the target mostly with the help of an E3 enzyme. In this process, Ubc9 not only forms a thioester bond with SUMO, but also interacts with SUMO noncovalently. Here, we show that this noncovalent interaction promotes the formation of short SUMO chains on targets such as Sp100 and HDAC4. We present a crystal structure of the noncovalent Ubc9-SUMO1 complex, showing that SUMO is located far from the E2 active site and resembles the noncovalent interaction site for ubiquitin on UbcH5c and Mms2. Structural comparison suggests a model for poly-sumoylation involving a mechanism analogous to Mms2-Ubc13-mediated ubiquitin chain formation.

Noncovalent interaction between Ubc9 and SUMO promotes SUMO chain formation.,Knipscheer P, van Dijk WJ, Olsen JV, Mann M, Sixma TK EMBO J. 2007 Jun 6;26(11):2797-807. Epub 2007 May 10. PMID:17491593^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Nacerddine K, Lehembre F, Bhaumik M, Artus J, Cohen-Tannoudji M, Babinet C, Pandolfi PP, Dejean A. The SUMO pathway is essential for nuclear integrity and chromosome segregation in mice. Dev Cell. 2005 Dec;9(6):769-79. PMID:16326389 doi:S1534-5807(05)00389-8
↑ Park SW, Hu X, Gupta P, Lin YP, Ha SG, Wei LN. SUMOylation of Tr2 orphan receptor involves Pml and fine-tunes Oct4 expression in stem cells. Nat Struct Mol Biol. 2007 Jan;14(1):68-75. Epub 2006 Dec 24. PMID:17187077 doi:10.1038/nsmb1185
↑ Knipscheer P, van Dijk WJ, Olsen JV, Mann M, Sixma TK. Noncovalent interaction between Ubc9 and SUMO promotes SUMO chain formation. EMBO J. 2007 Jun 6;26(11):2797-807. Epub 2007 May 10. PMID:17491593

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2uyz"

@@ Line 1: / Line 1: @@
-==NON-COVALENT COMPLEX BETWEEN UBC9 AND SUMO1==
+==Non-covalent complex between Ubc9 and SUMO1==
 <StructureSection load='2uyz' size='340' side='right' caption='[[2uyz]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
 == Structural highlights ==
@@ Line 6: / Line 7: @@
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1u9a|1u9a]], [[1u9b|1u9b]], [[1a5r|1a5r]], [[1tgz|1tgz]], [[1wyw|1wyw]], [[1y8r|1y8r]], [[1z5s|1z5s]], [[2asq|2asq]], [[2bf8|2bf8]], [[2g4d|2g4d]], [[2io2|2io2]], [[2iy0|2iy0]], [[2iy1|2iy1]]</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2uyz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uyz OCA], [http://pdbe.org/2uyz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2uyz RCSB], [http://www.ebi.ac.uk/pdbsum/2uyz PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2uyz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uyz OCA], [http://pdbe.org/2uyz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2uyz RCSB], [http://www.ebi.ac.uk/pdbsum/2uyz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2uyz ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 14: / Line 15: @@
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/uy/2uyz_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/uy/2uyz_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
@@ Line 40: / Line 41: @@
 [[Category: Lk3 transgenic mice]]
 [[Category: Ubiquitin--protein ligase]]
-[[Category: Dijk, W J.Van]]
+[[Category: Dijk, W J.van]]
 [[Category: Knipscheer, P]]
 [[Category: Mann, M]]

2uyz

From Proteopedia

Revision as of 06:52, 28 March 2018

Non-covalent complex between Ubc9 and SUMO1

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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