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2ouc

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Revision as of 09:09, 18 October 2017

Crystal structure of the MAP kinase binding domain of MKP5

Structural highlights

2ouc is a 2 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	2oud
Gene:	MKP5 (HUMAN)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[DUS10_HUMAN] Protein phosphatase involved in the inactivation of MAP kinases. Has a specificity for the MAPK11/MAPK12/MAPK13/MAPK14 subfamily.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

MAP kinase phosphatases (MKPs) have crucial roles in regulating the signaling activity of MAP kinases and are potential targets for drug discovery against human diseases. These enzymes contain a catalytic domain (CD) as well as a binding domain (BD) that help recognize the target MAP kinase. We report here the crystal structures at up to 2.2 A resolution of the BD and CD of human MKP5 and compare them to the known structures from other MKPs. Dramatic structural differences are observed between the BD of MKP5 and that of MKP3 determined previously by NMR. In particular, the cluster of positively charged residues that is important for MAP kinase binding is located in completely different positions in the two structures, with a distance of 25 A between them. Moreover, this cluster is alpha-helical in MKP5, while it forms a loop followed by a beta-strand in MKP3. These large structural differences could be associated with the distinct substrate preferences of these phosphatases, but further studies are needed to confirm this. The CD of MKP5 is observed in an active conformation, and two loops in the active site have backbone shifts of up to 5 A relative to the inactive CDs from other MKPs.

Crystal structure of the MAP kinase binding domain and the catalytic domain of human MKP5.,Tao X, Tong L Protein Sci. 2007 May;16(5):880-6. Epub 2007 Mar 30. PMID:17400920^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Zhang YY, Wu JW, Wang ZX. A Distinct Interaction Mode Revealed by the Crystal Structure of the Kinase p38alpha with the MAPK Binding Domain of the Phosphatase MKP5. Sci Signal. 2011 Dec 20;4(204):ra88. PMID:22375048 doi:10.1126/scisignal.2002241
↑ Tao X, Tong L. Crystal structure of the MAP kinase binding domain and the catalytic domain of human MKP5. Protein Sci. 2007 May;16(5):880-6. Epub 2007 Mar 30. PMID:17400920 doi:10.1110/ps.062712807

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2ouc"

Categories: Human | Tao, X | Tong, L | Hydrolase | Rhodanese fold

@@ Line 1: / Line 1: @@
 ==Crystal structure of the MAP kinase binding domain of MKP5==
 <StructureSection load='2ouc' size='340' side='right' caption='[[2ouc]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2oud|2oud]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MKP5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ouc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ouc OCA], [http://pdbe.org/2ouc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ouc RCSB], [http://www.ebi.ac.uk/pdbsum/2ouc PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ouc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ouc OCA], [http://pdbe.org/2ouc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ouc RCSB], [http://www.ebi.ac.uk/pdbsum/2ouc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ouc ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 28: / Line 29: @@
 </div>
 <div class="pdbe-citations 2ouc" style="background-color:#fffaf0;"></div>
-==See Also==
-*[[MAP kinase phosphatase|MAP kinase phosphatase]]
 == References ==
 <references/>

2ouc

From Proteopedia

Revision as of 09:09, 18 October 2017

Crystal structure of the MAP kinase binding domain of MKP5

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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