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1dm0

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[[Image:1dm0.gif|left|200px]]
[[Image:1dm0.gif|left|200px]]
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{{Structure
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The line below this paragraph, containing "STRUCTURE_1dm0", creates the "Structure Box" on the page.
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] </span>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1dm0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dm0 OCA], [http://www.ebi.ac.uk/pdbsum/1dm0 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1dm0 RCSB]</span>
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'''SHIGA TOXIN'''
'''SHIGA TOXIN'''
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[[Category: Protein complex]]
[[Category: Protein complex]]
[[Category: Shigella dysenteriae]]
[[Category: Shigella dysenteriae]]
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[[Category: rRNA N-glycosylase]]
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[[Category: RRNA N-glycosylase]]
[[Category: Chernaia, M M.]]
[[Category: Chernaia, M M.]]
[[Category: Fraser, M E.]]
[[Category: Fraser, M E.]]
[[Category: James, M N.]]
[[Category: James, M N.]]
[[Category: Kozlov, Y V.]]
[[Category: Kozlov, Y V.]]
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[[Category: ab5 structure]]
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[[Category: Ab5 structure]]
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[[Category: active site]]
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[[Category: Active site]]
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[[Category: blocking]]
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[[Category: Blocking]]
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[[Category: polypeptide some]]
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[[Category: Polypeptide some]]
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Revision as of 11:00, 2 May 2008

Template:STRUCTURE 1dm0

SHIGA TOXIN


Overview

Shigella dysenteriae is the pathogen responsible for the severe form of dysentery in humans. It produces Shiga toxin, the prototype of a family of closely related bacterial protein toxins. We have determined the structure of the holotoxin, an AB5 hexamer, by X-ray crystallography. The five B subunits form a pentameric ring, encircling a helix at the carboxy terminus of the A subunit. The A subunit interacts with the B pentamer via this C-terminal helix and a four-stranded mixed beta-sheet. The fold of the rest of the A subunit is similar to that of the A chain of the plant toxin ricin; both are N-glycosidases. However, the active site in the bacterial holotoxin is blocked by a segment of polypeptide chain. These residues of the A subunit would be released as part of the activation mechanism of the toxin.

About this Structure

1DM0 is a Protein complex structure of sequences from Shigella dysenteriae. Full crystallographic information is available from OCA.

Reference

Crystal structure of the holotoxin from Shigella dysenteriae at 2.5 A resolution., Fraser ME, Chernaia MM, Kozlov YV, James MN, Nat Struct Biol. 1994 Jan;1(1):59-64. PMID:7656009 Page seeded by OCA on Fri May 2 14:00:12 2008

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