2ckk

From Proteopedia

(Difference between revisions)

Revision as of 07:19, 29 March 2017

High resolution crystal structure of the human kin17 C-terminal domain containing a kow motif

Structural highlights

2ckk is a 1 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The human KIN17 protein is an essential nuclear protein conserved from yeast to human and expressed ubiquitously in mammals. Suppression of Rts2, the yeast equivalent of gene KIN17, renders the cells unviable, and silencing the human KIN17 gene slows cell growth dramatically. Moreover, the human gene KIN17 is up-regulated following exposure to ionizing radiations and UV light, depending on the integrity of the human global genome repair machinery. Its ectopic over-expression blocks S-phase progression by inhibiting DNA synthesis. The C-terminal region of human KIN17 is crucial for this anti-proliferation effect. Its high-resolution structure, presented here, reveals a tandem of SH3-like subdomains. This domain binds to ribonucleotide homopolymers with the same preferences as the whole protein. Analysis of its structure complexed with tungstate shows structural variability within the domain. The interaction with tungstate is mediated by several lysine residues located within a positively charged groove at the interface between the two subdomains. This groove could be the site of interaction with RNA, since mutagenesis of two of these highly conserved lysine residue weakens RNA binding.

A tandem of SH3-like domains participates in RNA binding in KIN17, a human protein activated in response to genotoxics.,le Maire A, Schiltz M, Stura EA, Pinon-Lataillade G, Couprie J, Moutiez M, Gondry M, Angulo JF, Zinn-Justin S J Mol Biol. 2006 Dec 8;364(4):764-76. Epub 2006 Sep 16. PMID:17045609^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ le Maire A, Schiltz M, Stura EA, Pinon-Lataillade G, Couprie J, Moutiez M, Gondry M, Angulo JF, Zinn-Justin S. A tandem of SH3-like domains participates in RNA binding in KIN17, a human protein activated in response to genotoxics. J Mol Biol. 2006 Dec 8;364(4):764-76. Epub 2006 Sep 16. PMID:17045609 doi:10.1016/j.jmb.2006.09.033

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2ckk"

@@ Line 1: / Line 1: @@
-==HIGH RESOLUTION CRYSTAL STRUCTURE OF THE HUMAN KIN17 C-TERMINAL DOMAIN CONTAINING A KOW MOTIF KIN17.==
+==High resolution crystal structure of the human kin17 C-terminal domain containing a kow motif==
 <StructureSection load='2ckk' size='340' side='right' caption='[[2ckk]], [[Resolution|resolution]] 1.45&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2ckk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CKK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CKK FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2ckk]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CKK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CKK FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ckk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ckk OCA], [http://pdbe.org/2ckk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ckk RCSB], [http://www.ebi.ac.uk/pdbsum/2ckk PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ckk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ckk OCA], [http://pdbe.org/2ckk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ckk RCSB], [http://www.ebi.ac.uk/pdbsum/2ckk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ckk ProSAT]</span></td></tr>
 </table>
 == Evolutionary Conservation ==
@@ Line 29: / Line 30: @@
 __TOC__
 </StructureSection>
-[[Category: Human]]
 [[Category: Angulo-Mora, J]]
 [[Category: Couprie, J]]
 [[Category: Gondry, M]]
-[[Category: Maire, A Le]]
+[[Category: Maire, A le]]
 [[Category: Pinon-Lataillade, G]]
 [[Category: Schiltz, M]]

2ckk

From Proteopedia

Revision as of 07:19, 29 March 2017

High resolution crystal structure of the human kin17 C-terminal domain containing a kow motif

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox