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2h27

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Revision as of 08:05, 18 October 2017

Crystal Structure of Escherichia coli SigmaE Region 4 Bound to its-35 Element DNA

Structural highlights

2h27 is a 6 chain structure with sequence from Ecoli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Gene:	rpoE (ECOLI)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[RPOE_ECOLI] Sigma factors are initiation factors that promote the attachment of RNA polymerase (RNAP) to specific initiation sites and are then released. Extracytoplasmic function (ECF) sigma-E controls the envelope stress response, responding to periplasmic protein stress, increased levels of periplasmic lipopolysaccharide (LPS) as well as heat shock and oxidative stress; it controls protein processing in the extracytoplasmic compartment. The 90 member regulon consists of the genes necessary for the synthesis and maintenance of both proteins and LPS of the outer membrane.^[1] ^[2] ^[3] ^[4] ^[5] ^[6]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The control of bacterial transcription initiation depends on a primary sigma factor for housekeeping functions, as well as alternative sigma factors that control regulons in response to environmental stresses. The largest and most diverse subgroup of alternative sigma factors, the group IV extracytoplasmic function sigma factors, directs the transcription of genes that regulate a wide variety of responses, including envelope stress and pathogenesis. We determined the 2.3-A resolution crystal structure of the -35 element recognition domain of a group IV sigma factor, Escherichia coli sigma(E)4, bound to its consensus -35 element, GGAACTT. Despite similar function and secondary structure, the primary and group IV sigma factors recognize their -35 elements using distinct mechanisms. Conserved sequence elements of the sigma(E) -35 element induce a DNA geometry characteristic of AA/TT-tract DNA, including a rigid, straight double-helical axis and a narrow minor groove. For this reason, the highly conserved AA in the middle of the GGAACTT motif is essential for -35 element recognition by sigma(E)4, despite the absence of direct protein-DNA interactions with these DNA bases. These principles of sigma(E)4/-35 element recognition can be applied to a wide range of other group IV sigma factors.

The structural basis for promoter -35 element recognition by the group IV sigma factors.,Lane WJ, Darst SA PLoS Biol. 2006 Sep;4(9):e269. PMID:16903784^[7]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Raina S, Missiakas D, Georgopoulos C. The rpoE gene encoding the sigma E (sigma 24) heat shock sigma factor of Escherichia coli. EMBO J. 1995 Mar 1;14(5):1043-55. PMID:7889935
↑ Rouviere PE, De Las Penas A, Mecsas J, Lu CZ, Rudd KE, Gross CA. rpoE, the gene encoding the second heat-shock sigma factor, sigma E, in Escherichia coli. EMBO J. 1995 Mar 1;14(5):1032-42. PMID:7889934
↑ Erickson JW, Gross CA. Identification of the sigma E subunit of Escherichia coli RNA polymerase: a second alternate sigma factor involved in high-temperature gene expression. Genes Dev. 1989 Sep;3(9):1462-71. PMID:2691330
↑ Missiakas D, Mayer MP, Lemaire M, Georgopoulos C, Raina S. Modulation of the Escherichia coli sigmaE (RpoE) heat-shock transcription-factor activity by the RseA, RseB and RseC proteins. Mol Microbiol. 1997 Apr;24(2):355-71. PMID:9159522
↑ De Las Penas A, Connolly L, Gross CA. The sigmaE-mediated response to extracytoplasmic stress in Escherichia coli is transduced by RseA and RseB, two negative regulators of sigmaE. Mol Microbiol. 1997 Apr;24(2):373-85. PMID:9159523
↑ Rhodius VA, Suh WC, Nonaka G, West J, Gross CA. Conserved and variable functions of the sigmaE stress response in related genomes. PLoS Biol. 2006 Jan;4(1):e2. PMID:16336047 doi:http://dx.doi.org/10.1371/journal.pbio.0040002
↑ Lane WJ, Darst SA. The structural basis for promoter -35 element recognition by the group IV sigma factors. PLoS Biol. 2006 Sep;4(9):e269. PMID:16903784 doi:10.1371/journal.pbio.0040269

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2h27"

@@ Line 1: / Line 1: @@
 ==Crystal Structure of Escherichia coli SigmaE Region 4 Bound to its-35 Element DNA==
 <StructureSection load='2h27' size='340' side='right' caption='[[2h27]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rpoE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2h27 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h27 OCA], [http://pdbe.org/2h27 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2h27 RCSB], [http://www.ebi.ac.uk/pdbsum/2h27 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2h27 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h27 OCA], [http://pdbe.org/2h27 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2h27 RCSB], [http://www.ebi.ac.uk/pdbsum/2h27 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2h27 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 28: / Line 29: @@
 </div>
 <div class="pdbe-citations 2h27" style="background-color:#fffaf0;"></div>
-==See Also==
-*[[Sigma factor|Sigma factor]]
 == References ==
 <references/>

2h27

From Proteopedia

Revision as of 08:05, 18 October 2017

Crystal Structure of Escherichia coli SigmaE Region 4 Bound to its-35 Element DNA

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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