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2cnw

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Revision as of 07:56, 23 May 2018

GDPALF4 complex of the SRP GTPases Ffh and FtsY

Structural highlights

2cnw is a 6 chain structure with sequence from Atcc 25104. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, , ,
Related:	1ffh, 1jpj, 1jpn, 1ls1, 1ng1, 1o87, 1okk, 1rj9, 1ry1, 2bqs, 2bqt, 2c03, 2c04, 2ffh, 2j45, 2j46, 2ng1, 3ng1
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[FTSY_THEAQ] Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Acts as a receptor for the complex formed by the signal recognition particle (SRP) and the ribosome-nascent chain (RNC) (By similarity).

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The signal recognition particle (SRP) GTPases Ffh and FtsY play a central role in co-translational targeting of proteins, assembling in a GTP-dependent manner to generate the SRP targeting complex at the membrane. A suite of residues in FtsY have been identified that are essential for the hydrolysis of GTP that accompanies disengagement. We have argued previously on structural grounds that this region mediates interactions that serve to activate the complex for disengagement and term it the activation region. We report here the structure of a complex of the SRP GTPases formed in the presence of GDP:AlF4. This complex accommodates the putative transition-state analog without undergoing significant change from the structure of the ground-state complex formed in the presence of the GTP analog GMPPCP. However, small shifts that do occur within the shared catalytic chamber may be functionally important. Remarkably, an external nucleotide interaction site was identified at the activation region, revealed by an unexpected contaminating GMP molecule bound adjacent to the catalytic chamber. This site exhibits conserved sequence and structural features that suggest a direct interaction with RNA plays a role in regulating the activity of the SRP targeting complex.

Structure of a GDP:AlF4 complex of the SRP GTPases Ffh and FtsY, and identification of a peripheral nucleotide interaction site.,Focia PJ, Gawronski-Salerno J, Coon JS 5th, Freymann DM J Mol Biol. 2006 Jul 14;360(3):631-43. Epub 2006 May 26. PMID:16780874^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Focia PJ, Gawronski-Salerno J, Coon JS 5th, Freymann DM. Structure of a GDP:AlF4 complex of the SRP GTPases Ffh and FtsY, and identification of a peripheral nucleotide interaction site. J Mol Biol. 2006 Jul 14;360(3):631-43. Epub 2006 May 26. PMID:16780874 doi:10.1016/j.jmb.2006.05.031

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2cnw"

@@ Line 1: / Line 1: @@
-==GDPALF4 COMPLEX OF THE SRP GTPASES FFH AND FTSY==
+==GDPALF4 complex of the SRP GTPases Ffh and FtsY==
 <StructureSection load='2cnw' size='340' side='right' caption='[[2cnw]], [[Resolution|resolution]] 2.39&Aring;' scene=''>
 == Structural highlights ==
@@ Line 5: / Line 6: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=5GP:GUANOSINE-5-MONOPHOSPHATE'>5GP</scene>, <scene name='pdbligand=ALF:TETRAFLUOROALUMINATE+ION'>ALF</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1ffh|1ffh]], [[1jpj|1jpj]], [[1jpn|1jpn]], [[1ls1|1ls1]], [[1ng1|1ng1]], [[1o87|1o87]], [[1okk|1okk]], [[1rj9|1rj9]], [[1ry1|1ry1]], [[2bqs|2bqs]], [[2bqt|2bqt]], [[2c03|2c03]], [[2c04|2c04]], [[2ffh|2ffh]], [[2j45|2j45]], [[2j46|2j46]], [[2ng1|2ng1]], [[3ng1|3ng1]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cnw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cnw OCA], [http://pdbe.org/2cnw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2cnw RCSB], [http://www.ebi.ac.uk/pdbsum/2cnw PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cnw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cnw OCA], [http://pdbe.org/2cnw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2cnw RCSB], [http://www.ebi.ac.uk/pdbsum/2cnw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2cnw ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 13: / Line 14: @@
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cn/2cnw_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cn/2cnw_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>

2cnw

From Proteopedia

Revision as of 07:56, 23 May 2018

GDPALF4 complex of the SRP GTPases Ffh and FtsY

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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