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1kix

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Revision as of 21:18, 24 January 2018

Dimeric Structure of the O. nova Telomere End Binding Protein Alpha Subunit with Bound ssDNA

Structural highlights

1kix is a 2 chain structure with sequence from Ciliate. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	1otc, 1jb7, 1k8g
Gene:	MAC-56A (Ciliate)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[TEBA_OXYNO] May function as protective capping of the single-stranded telomeric overhang. May also participate in telomere length regulation during DNA replication. Binds specifically to the T4G4-containing extension on the 3'strand and protects this region of the telomere from nuclease digestion and chemical modification.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Telomeres are the specialized protein--DNA complexes that cap and protect the ends of linear eukaryotic chromosomes. The extreme 3' end of the telomeric DNA in Oxytricha nova is bound by a two-subunit sequence-specific and 3' end-specific protein called the telomere end-binding protein (OnTEBP). Here we describe the crystal structure of the alpha-subunit of OnTEBP in complex with T4G4 single-stranded telomeric DNA. This structure shows an (alpha--ssDNA)2 homodimer with a large approximately 7,000 A2 protein--protein interface in which the domains of alpha are rearranged extensively from their positions in the structure of an alpha--beta--ssDNA ternary complex. The (alpha--ssDNA)2 complex can bind two telomeres on opposite sides of the dimer and, thus, acts as a protein mediator of telomere--telomere associations. The structures of the (alpha--ssDNA)2 dimer presented here and the previously described alpha--beta--ssDNA complex demonstrate that OnTEBP forms multiple telomeric complexes that potentially mediate the assembly and disassembly of higher order telomeric structures.

Dimeric structure of the Oxytricha nova telomere end-binding protein alpha-subunit bound to ssDNA.,Peersen OB, Ruggles JA, Schultz SC Nat Struct Biol. 2002 Mar;9(3):182-7. PMID:11836536^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Peersen OB, Ruggles JA, Schultz SC. Dimeric structure of the Oxytricha nova telomere end-binding protein alpha-subunit bound to ssDNA. Nat Struct Biol. 2002 Mar;9(3):182-7. PMID:11836536 doi:http://dx.doi.org/10.1038/nsb761

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1kix"

@@ Line 1: / Line 1: @@
 ==Dimeric Structure of the O. nova Telomere End Binding Protein Alpha Subunit with Bound ssDNA==
 <StructureSection load='1kix' size='340' side='right' caption='[[1kix]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
@@ Line 6: / Line 7: @@
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1otc|1otc]], [[1jb7|1jb7]], [[1k8g|1k8g]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MAC-56A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=200597 Ciliate])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kix OCA], [http://pdbe.org/1kix PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1kix RCSB], [http://www.ebi.ac.uk/pdbsum/1kix PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kix OCA], [http://pdbe.org/1kix PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1kix RCSB], [http://www.ebi.ac.uk/pdbsum/1kix PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1kix ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 14: / Line 15: @@
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ki/1kix_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ki/1kix_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>

1kix

From Proteopedia

Revision as of 21:18, 24 January 2018

Dimeric Structure of the O. nova Telomere End Binding Protein Alpha Subunit with Bound ssDNA

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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