1dt0

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[[Image:1dt0.gif|left|200px]]
[[Image:1dt0.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1dt0 |SIZE=350|CAPTION= <scene name='initialview01'>1dt0</scene>, resolution 2.1&Aring;
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The line below this paragraph, containing "STRUCTURE_1dt0", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Superoxide_dismutase Superoxide dismutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.15.1.1 1.15.1.1] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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|DOMAIN=
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{{STRUCTURE_1dt0| PDB=1dt0 | SCENE= }}
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|RELATEDENTRY=[[1ar4|1ar4]], [[1ids|1ids]], [[1isb|1isb]], [[1isc|1isc]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1dt0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dt0 OCA], [http://www.ebi.ac.uk/pdbsum/1dt0 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1dt0 RCSB]</span>
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}}
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'''CLONING, SEQUENCE, AND CRYSTALLOGRAPHIC STRUCTURE OF RECOMBINANT IRON SUPEROXIDE DISMUTASE FROM PSEUDOMONAS OVALIS'''
'''CLONING, SEQUENCE, AND CRYSTALLOGRAPHIC STRUCTURE OF RECOMBINANT IRON SUPEROXIDE DISMUTASE FROM PSEUDOMONAS OVALIS'''
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[[Category: Huang, J.]]
[[Category: Huang, J.]]
[[Category: Stoddard, B L.]]
[[Category: Stoddard, B L.]]
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[[Category: pseudomonas ovali]]
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[[Category: Pseudomonas ovali]]
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[[Category: superoxide dismutase]]
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[[Category: Superoxide dismutase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 14:14:15 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:48:08 2008''
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Revision as of 11:14, 2 May 2008

Template:STRUCTURE 1dt0

CLONING, SEQUENCE, AND CRYSTALLOGRAPHIC STRUCTURE OF RECOMBINANT IRON SUPEROXIDE DISMUTASE FROM PSEUDOMONAS OVALIS


Overview

The gene encoding the iron-dependent superoxide dismutase from Pseudomonas ovalis was cloned from a genomic library and sequenced. The ORF differs from the previously published protein sequence, which was used for the original structure determination, at 16 positions. The differences include three additional inserted residues, one deleted residue and 12 point substitutions. The gene was subcloned and the recombinant protein overexpressed, purified and crystallized in a trigonal space group. The structure was determined by molecular replacement and was refined to 2.1 A resolution.

About this Structure

1DT0 is a Single protein structure of sequence from Pseudomonas putida. Full crystallographic information is available from OCA.

Reference

Cloning, sequence and crystallographic structure of recombinant iron superoxide dismutase from Pseudomonas ovalis., Bond CJ, Huang J, Hajduk R, Flick KE, Heath PJ, Stoddard BL, Acta Crystallogr D Biol Crystallogr. 2000 Nov;56(Pt 11):1359-66. PMID:11053832 Page seeded by OCA on Fri May 2 14:14:15 2008

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