This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

2vgq

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 13:34, 15 March 2017

Crystal Structure of Human IPS-1 CARD

Structural highlights

2vgq is a 1 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Related:	1mh3, 1hsj, 1y4c, 1ezo, 1fqc, 1anf, 1lls, 3mbp, 1zkb, 1mpb, 1dmb, 1ezp, 1ez9, 1zjl, 2h25, 1jvx, 1n3x, 1nl5, 1a7l, 1r6z, 1zmg, 1t0k, 1mh4, 1jw5, 1fqa, 1iud, 1ziu, 1mg1, 1mdp, 1fqb, 1jw4, 1omp, 1n3w, 1nmu, 1mpd, 1ytv, 1mdq, 1lax, 1peb, 1mpc, 1jvy, 1fqd, 1svx, 2d21
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

BACKGROUND: IPS-1/MAVS/VISA/Cardif is an adaptor protein that plays a crucial role in the induction of interferons in response to viral infection. In the initial stage of the intracellular antiviral response two RNA helicases, retinoic acid inducible gene-I (RIG-I) and melanoma differentiation-association gene 5 (MDA5), are independently able to bind viral RNA in the cytoplasm. The 62 kDa protein IPS-1/MAVS/VISA/Cardif contains an N-terminal caspase activation and recruitment (CARD) domain that associates with the CARD regions of RIG-I and MDA5, ultimately leading to the induction of type I interferons. As a first step towards understanding the molecular basis of this important adaptor protein we have undertaken structural studies of the IPS-1 MAVS/VISA/Cardif CARD region. RESULTS: The crystal structure of human IPS-1/MAVS/VISA/Cardif CARD has been determined to 2.1A resolution. The protein was expressed and crystallized as a maltose-binding protein (MBP) fusion protein. The MBP and IPS-1 components each form a distinct domain within the structure. IPS-1/MAVS/VISA/Cardif CARD adopts a characteristic six-helix bundle with a Greek-key topology and, in common with a number of other known CARD structures, contains two major polar surfaces on opposite sides of the molecule. One face has a surface-exposed, disordered tryptophan residue that may explain the poor solubility of untagged expression constructs. CONCLUSION: The IPS-1/MAVS/VISA/Cardif CARD domain adopts the classic CARD fold with an asymmetric surface charge distribution that is typical of CARD domains involved in homotypic protein-protein interactions. The location of the two polar areas on IPS-1/MAVS/VISA/Cardif CARD suggest possible types of associations that this domain makes with the two CARD domains of MDA5 or RIG-I. The N-terminal CARD domains of RIG-I and MDA5 share greatest sequence similarity with IPS-1/MAVS/VISA/Cardif CARD and this has allowed modelling of their structures. These models show a very different charge profile for the equivalent surfaces compared to IPS-1/MAVS/VISA/Cardif CARD.

Crystal structure of human IPS-1/MAVS/VISA/Cardif caspase activation recruitment domain.,Potter JA, Randall RE, Taylor GL BMC Struct Biol. 2008 Feb 28;8:11. PMID:18307765^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Potter JA, Randall RE, Taylor GL. Crystal structure of human IPS-1/MAVS/VISA/Cardif caspase activation recruitment domain. BMC Struct Biol. 2008 Feb 28;8:11. PMID:18307765 doi:1472-6807-8-11

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2vgq"

@@ Line 1: / Line 1: @@
-==CRYSTAL STRUCTURE OF HUMAN IPS-1 CARD==
+==Crystal Structure of Human IPS-1 CARD==
 <StructureSection load='2vgq' size='340' side='right' caption='[[2vgq]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2vgq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VGQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VGQ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2vgq]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VGQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VGQ FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MTT:MALTOTETRAOSE'>MTT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1mh3|1mh3]], [[1hsj|1hsj]], [[1y4c|1y4c]], [[1ezo|1ezo]], [[1fqc|1fqc]], [[1anf|1anf]], [[1lls|1lls]], [[3mbp|3mbp]], [[1zkb|1zkb]], [[1mpb|1mpb]], [[1dmb|1dmb]], [[1ezp|1ezp]], [[1ez9|1ez9]], [[1zjl|1zjl]], [[2h25|2h25]], [[1jvx|1jvx]], [[1n3x|1n3x]], [[1nl5|1nl5]], [[1a7l|1a7l]], [[1r6z|1r6z]], [[1zmg|1zmg]], [[1t0k|1t0k]], [[1mh4|1mh4]], [[1jw5|1jw5]], [[1fqa|1fqa]], [[1iud|1iud]], [[1ziu|1ziu]], [[1mg1|1mg1]], [[1mdp|1mdp]], [[1fqb|1fqb]], [[1jw4|1jw4]], [[1omp|1omp]], [[1n3w|1n3w]], [[1nmu|1nmu]], [[1mpd|1mpd]], [[1ytv|1ytv]], [[1mdq|1mdq]], [[1lax|1lax]], [[1peb|1peb]], [[1mpc|1mpc]], [[1jvy|1jvy]], [[1fqd|1fqd]], [[1svx|1svx]], [[2d21|2d21]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vgq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vgq OCA], [http://pdbe.org/2vgq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2vgq RCSB], [http://www.ebi.ac.uk/pdbsum/2vgq PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vgq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vgq OCA], [http://pdbe.org/2vgq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2vgq RCSB], [http://www.ebi.ac.uk/pdbsum/2vgq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2vgq ProSAT]</span></td></tr>
 </table>
 == Evolutionary Conservation ==
@@ Line 30: / Line 31: @@
 __TOC__
 </StructureSection>
-[[Category: Human]]
 [[Category: Potter, J A]]
 [[Category: Randall, R E]]

2vgq

From Proteopedia

Revision as of 13:34, 15 March 2017

Crystal Structure of Human IPS-1 CARD

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox