1dw4
From Proteopedia
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[[Image:1dw4.gif|left|200px]] | [[Image:1dw4.gif|left|200px]] | ||
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| - | + | {{STRUCTURE_1dw4| PDB=1dw4 | SCENE= }} | |
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'''NMR STRUCTURE OF OMEGA-CONOTOXIN MVIIA: CONSTRAINTS ON DISULPHIDE BRIDGES''' | '''NMR STRUCTURE OF OMEGA-CONOTOXIN MVIIA: CONSTRAINTS ON DISULPHIDE BRIDGES''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1DW4 is a [[Single protein]] structure | + | 1DW4 is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DW4 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Lefevre, J F.]] | [[Category: Lefevre, J F.]] | ||
[[Category: Sirockin, F.]] | [[Category: Sirockin, F.]] | ||
| - | [[Category: | + | [[Category: Calcium channel]] |
| - | [[Category: | + | [[Category: Conformational exchange]] |
| - | [[Category: | + | [[Category: Conotoxin]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 14:21:06 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 11:21, 2 May 2008
NMR STRUCTURE OF OMEGA-CONOTOXIN MVIIA: CONSTRAINTS ON DISULPHIDE BRIDGES
Overview
omega-Conotoxin MVIIA is a 25-residue, disulfide-bridged polypeptide from the venom of the sea snail Conus magus that binds to neuronal N-type calcium channels. It forms a compact folded structure, presenting a loop between Cys8 and Cys15 that contains a set of residues critical for its binding. The loop does not have a unique defined structure, nor is it intrinsically flexible. Broadening of a subset of resonances in the NMR spectrum at low temperature, anomalous temperature dependence of the chemical shifts of some resonances, and exchange contributions to J(0) from (13)C relaxation measurements reveal that conformational exchange affects the residues in this loop. The effects of this exchange on the calculated structure of omega-conotoxin MVIIA are discussed. The exchange appears to be associated with a change in the conformation of the disulfide bridge Cys8-Cys20. The implications for the use of the omega-conotoxins as a scaffold for carrying other functions is discussed.
About this Structure
1DW4 is a Single protein structure. Full crystallographic information is available from OCA.
Reference
Structural and dynamic characterization of omega-conotoxin MVIIA: the binding loop exhibits slow conformational exchange., Atkinson RA, Kieffer B, Dejaegere A, Sirockin F, Lefevre JF, Biochemistry. 2000 Apr 11;39(14):3908-19. PMID:10747778 Page seeded by OCA on Fri May 2 14:21:06 2008
