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3hwj

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Revision as of 07:49, 12 December 2018

Crystal structure of the second PHR domain of Mouse Myc-binding protein 2 (MYCBP-2)

Structural highlights

3hwj is a 2 chain structure with sequence from Lk3 transgenic mice. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
NonStd Res:
Related:	3gbw
Gene:	Mycbp2, Pam, Phr1 (LK3 transgenic mice)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[MYCB2_MOUSE] Probable E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins. May have a role during synaptogenesis; candidate for respiratory distress and ventilatory disorders that arise from defective neuronal control of breathing. May function as a facilitator or regulator of transcriptional activation by MYC.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

PHR [PAM (protein associated with Myc)-HIW (Highwire)-RPM-1 (regulator of presynaptic morphology 1)] proteins are conserved, large multi-domain E3 ubiquitin ligases with modular architecture. PHR proteins presynaptically control synaptic growth and axon guidance and postsynaptically regulate endocytosis of glutamate receptors. Dysfunction of neuronal ubiquitin-mediated proteasomal degradation is implicated in various neurodegenerative diseases. PHR proteins are characterized by the presence of two PHR domains near the N-terminus, which are essential for proper localization and function. Structures of both the first and second PHR domains of Mus musculus (mouse) Phr1 (MYC binding protein 2, Mycbp2) have been determined, revealing a novel beta sandwich fold composed of 11 antiparallel beta-strands. Conserved loops decorate the apical side of the first PHR domain (MmPHR1), yielding a distinct conserved surface feature. The surface of the second PHR domain (MmPHR2), in contrast, lacks significant conservation. Importantly, the structure of MmPHR1 provides insights into a loss-of-function mutation, Gly1092-->Glu, observed in the Caenorhabditis elegans ortholog RPM-1.

Structures of PHR domains from Mus musculus Phr1 (Mycbp2) explain the loss-of-function mutation (Gly1092-->Glu) of the C. elegans ortholog RPM-1.,Sampathkumar P, Ozyurt SA, Miller SA, Bain KT, Rutter ME, Gheyi T, Abrams B, Wang Y, Atwell S, Luz JG, Thompson DA, Wasserman SR, Emtage JS, Park EC, Rongo C, Jin Y, Klemke RL, Sauder JM, Burley SK J Mol Biol. 2010 Apr 9;397(4):883-92. Epub 2010 Feb 13. PMID:20156452^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Burgess RW, Peterson KA, Johnson MJ, Roix JJ, Welsh IC, O'Brien TP. Evidence for a conserved function in synapse formation reveals Phr1 as a candidate gene for respiratory failure in newborn mice. Mol Cell Biol. 2004 Feb;24(3):1096-105. PMID:14729956
↑ Sampathkumar P, Ozyurt SA, Miller SA, Bain KT, Rutter ME, Gheyi T, Abrams B, Wang Y, Atwell S, Luz JG, Thompson DA, Wasserman SR, Emtage JS, Park EC, Rongo C, Jin Y, Klemke RL, Sauder JM, Burley SK. Structures of PHR domains from Mus musculus Phr1 (Mycbp2) explain the loss-of-function mutation (Gly1092-->Glu) of the C. elegans ortholog RPM-1. J Mol Biol. 2010 Apr 9;397(4):883-92. Epub 2010 Feb 13. PMID:20156452 doi:10.1016/j.jmb.2010.02.017

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3hwj"

@@ Line 1: / Line 1: @@
 ==Crystal structure of the second PHR domain of Mouse Myc-binding protein 2 (MYCBP-2)==
 <StructureSection load='3hwj' size='340' side='right' caption='[[3hwj]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
@@ Line 7: / Line 8: @@
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3gbw|3gbw]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Mycbp2, Pam, Phr1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hwj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hwj OCA], [http://pdbe.org/3hwj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3hwj RCSB], [http://www.ebi.ac.uk/pdbsum/3hwj PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hwj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hwj OCA], [http://pdbe.org/3hwj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3hwj RCSB], [http://www.ebi.ac.uk/pdbsum/3hwj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3hwj ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 15: / Line 16: @@
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hw/3hwj_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hw/3hwj_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
@@ Line 50: / Line 51: @@
 [[Category: Sauder, J M]]
 [[Category: Wasserman, S R]]
+[[Category: Alternative splicing]]
 [[Category: Ligase]]
 [[Category: Metal-binding]]
@@ Line 67: / Line 69: @@
 [[Category: Transcription regulation]]
 [[Category: Ubl conjugation pathway]]
+[[Category: Zinc]]
 [[Category: Zinc-finger]]

3hwj

From Proteopedia

Revision as of 07:49, 12 December 2018

Crystal structure of the second PHR domain of Mouse Myc-binding protein 2 (MYCBP-2)

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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