1e0x

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[[Image:1e0x.jpg|left|200px]]
[[Image:1e0x.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 1e0x |SIZE=350|CAPTION= <scene name='initialview01'>1e0x</scene>, resolution 1.65&Aring;
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The line below this paragraph, containing "STRUCTURE_1e0x", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:X2f+Binding+Site+For+Chain+A'>AC1</scene>, <scene name='pdbsite=AC2:Xys+Binding+Site+For+Chain+A'>AC2</scene>, <scene name='pdbsite=AC3:X2f+Binding+Site+For+Chain+B'>AC3</scene>, <scene name='pdbsite=AC4:Xys+Binding+Site+For+Chain+B'>AC4</scene>, <scene name='pdbsite=ACA:Catalytic+Acid/Base+Chain+A'>ACA</scene>, <scene name='pdbsite=ACB:Catalytic+Acid/Base+Chain+B'>ACB</scene>, <scene name='pdbsite=NUA:Catalytic+Nucleophile+Chain+A'>NUA</scene> and <scene name='pdbsite=NUB:Catalytic+Nucleophile+Chain+B'>NUB</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=X2F:2-DEOXY-2-FLUORO+XYLOPYRANOSE'>X2F</scene>, <scene name='pdbligand=XYP:BETA-D-XYLOPYRANOSE'>XYP</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1e0x| PDB=1e0x | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1e0x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e0x OCA], [http://www.ebi.ac.uk/pdbsum/1e0x PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1e0x RCSB]</span>
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}}
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'''XYLANASE 10A FROM SREPTOMYCES LIVIDANS. XYLOBIOSYL-ENZYME INTERMEDIATE AT 1.65 A'''
'''XYLANASE 10A FROM SREPTOMYCES LIVIDANS. XYLOBIOSYL-ENZYME INTERMEDIATE AT 1.65 A'''
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[[Category: Morosoli, R.]]
[[Category: Morosoli, R.]]
[[Category: Shareck, F.]]
[[Category: Shareck, F.]]
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[[Category: glycoside hydrolase family 10]]
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[[Category: Glycoside hydrolase family 10]]
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[[Category: glycosyl-enzyme intermediate]]
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[[Category: Glycosyl-enzyme intermediate]]
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[[Category: xylan degradation]]
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[[Category: Xylan degradation]]
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[[Category: xylanase]]
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[[Category: Xylanase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 14:31:25 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:52:48 2008''
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Revision as of 11:31, 2 May 2008

Image:1e0x.jpg

Template:STRUCTURE 1e0x

XYLANASE 10A FROM SREPTOMYCES LIVIDANS. XYLOBIOSYL-ENZYME INTERMEDIATE AT 1.65 A


Overview

Endoxylanases are a group of enzymes that hydrolyze the beta-1, 4-linked xylose backbone of xylans. They are predominantly found in two discrete sequence families known as glycoside hydrolase families 10 and 11. The Streptomyces lividans xylanase Xyl10A is a family 10 enzyme, the native structure of which has previously been determined by x-ray crystallography at a 2.6 A resolution (Derewenda, U., Swenson, L., Green, R., Wei, Y., Morosoli, R., Shareck, F., Kluepfel, D., and Derewenda, Z. S. (1994) J. Biol. Chem. 269, 20811-20814). Here, we report the native structure of Xyl10A refined at a resolution of 1.2 A, which reveals many features such as the rare occurrence of a discretely disordered disulfide bond between residues Cys-168 and Cys-201. In order to investigate substrate binding and specificity in glycoside hydrolase family 10, the covalent xylobiosyl enzyme and the covalent cellobiosyl enzyme intermediates of Xyl10A were trapped through the use of appropriate 2-fluoroglycosides. The alpha-linked intermediate with the nucleophile, Glu-236, is in a (4)C(1) chair conformation as previously observed in the family 10 enzyme Cex from Cellulomonas fimi (Notenboom, V., Birsan, C., Warren, R. A. J., Withers, S. G., and Rose, D. R. (1998) Biochemistry 37, 4751-4758). The different interactions of Xyl10A with the xylobiosyl and cellobiosyl moieties, notably conformational changes in the -2 and -1 subsites, together with the observed kinetics on a range of aryl glycosides, shed new light on substrate specificity in glycoside hydrolase family 10.

About this Structure

1E0X is a Single protein structure of sequence from Streptomyces lividans. Full crystallographic information is available from OCA.

Reference

Substrate specificity in glycoside hydrolase family 10. Structural and kinetic analysis of the Streptomyces lividans xylanase 10A., Ducros V, Charnock SJ, Derewenda U, Derewenda ZS, Dauter Z, Dupont C, Shareck F, Morosoli R, Kluepfel D, Davies GJ, J Biol Chem. 2000 Jul 28;275(30):23020-6. PMID:10930426 Page seeded by OCA on Fri May 2 14:31:25 2008

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