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2v4r

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Revision as of 08:08, 12 September 2018

Non-productive complex of the Y-family DNA polymerase Dpo4 with dGTP skipping the M1dG adduct to pair with the next template cytosine

Structural highlights

2v4r is a 3 chain structure with sequence from Sulso. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
NonStd Res:
Related:	2asl, 1s0m, 2jeg, 1rys, 2j6t, 1n56, 2atl, 1jx4, 2c22, 2va2, 1s97, 2agq, 2jej, 2ago, 2asj, 2asd, 1n48, 1jxl, 2bq3, 2j6u, 2uvu, 2agp, 2c2r, 1s0n, 1s0o, 2jef, 2uvw, 2bqr, 2jei, 2bqu, 1s9f, 2au0, 2c28, 2v9w, 2c2e, 1ryr, 2c2d, 2uvv, 1s10, 2br0, 2j6s, 2uvr, 2va3, 2v4q, 2v4s, 2v4t
Activity:	DNA-directed DNA polymerase, with EC number 2.7.7.7
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[DPO4_SULSO] Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. It is involved in translesional synthesis.[HAMAP-Rule:MF_01113]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Oxidative stress can induce the formation of reactive electrophiles, such as DNA peroxidation products, e.g. base propenals, and lipid peroxidation products, e.g. malondialdehyde. Base propenals and malondialdehyde react with DNA to form adducts, including 3-(2'-deoxy-ss-D-erythro-pentofuranosyl)-pyrimido[1,2-alpha]purin-10(3H)-o ne (M1dG). When paired opposite cytosine in duplex DNA at physiological pH, M1dG undergoes ring opening to form N2-(3-oxo-1-propenyl)-dG (N2-OPdG). Previous work has shown that M1dG is mutagenic in bacteria and mammalian cells and that its mutagenicity in Escherichia coli is dependent on induction of the SOS response, indicating a role for translesion DNA polymerases in the bypass of M1dG. To probe the mechanism by which translesion polymerases bypass M1dG, kinetic and structural studies were conducted with a model Y-family DNA polymerase, Dpo4 from Sulfolobus solfataricus. Steady-state incorporation of dNTPs opposite M1dG was reduced 260- to 2,900-fold and exhibited a preference for dATP incorporation. LC-MS/MS analysis of the full-length extension products revealed a spectrum of products arising principally by incorporation of dC or dA opposite M1dG followed by partial or full-length extension. A greater proportion of -1 deletions were observed when dT was positioned 5' of M1dG. Two crystal structures were solved, including a "Type II" frameshift deletion complex and another complex with Dpo4 bound to a dC:M1dG pair located in the post-insertion context. Importantly, M1dG was in the ring-closed state in both structures and in the structure with dC opposite M1dG, the dC residue moved out of the Dpo4 active site, into the minor groove. The results are consistent with the reported mutagenicity of M1dG and illustrate how the lesion may affect replication events.

Structural and Functional Analysis of Sulfolobus solfataricus Y-family DNA polymerase Dpo4-catalyzed Bypass of the Malondialdehyde-deoxyguanosine Adduct.,Eoff RL, Stafford JB, Szekely J, Rizzo CJ, Egli M, Guengerich FP, Marnett LJ Biochemistry. 2009 Jun 4. PMID:19492857^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Eoff RL, Stafford JB, Szekely J, Rizzo CJ, Egli M, Guengerich FP, Marnett LJ. Structural and Functional Analysis of Sulfolobus solfataricus Y-family DNA polymerase Dpo4-catalyzed Bypass of the Malondialdehyde-deoxyguanosine Adduct. Biochemistry. 2009 Jun 4. PMID:19492857 doi:10.1021/bi9003588

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2v4r"

@@ Line 1: / Line 1: @@
-==NON-PRODUCTIVE COMPLEX OF THE Y-FAMILY DNA POLYMERASE DPO4 WITH DGTP SKIPPING THE M1DG ADDUCT TO PAIR WITH THE NEXT TEMPLATE CYTOSINE==
+==Non-productive complex of the Y-family DNA polymerase Dpo4 with dGTP skipping the M1dG adduct to pair with the next template cytosine==
 <StructureSection load='2v4r' size='340' side='right' caption='[[2v4r]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
 == Structural highlights ==
@@ Line 7: / Line 8: @@
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2asl|2asl]], [[1s0m|1s0m]], [[2jeg|2jeg]], [[1rys|1rys]], [[2j6t|2j6t]], [[1n56|1n56]], [[2atl|2atl]], [[1jx4|1jx4]], [[2c22|2c22]], [[2va2|2va2]], [[1s97|1s97]], [[2agq|2agq]], [[2jej|2jej]], [[2ago|2ago]], [[2asj|2asj]], [[2asd|2asd]], [[1n48|1n48]], [[1jxl|1jxl]], [[2bq3|2bq3]], [[2j6u|2j6u]], [[2uvu|2uvu]], [[2agp|2agp]], [[2c2r|2c2r]], [[1s0n|1s0n]], [[1s0o|1s0o]], [[2jef|2jef]], [[2uvw|2uvw]], [[2bqr|2bqr]], [[2jei|2jei]], [[2bqu|2bqu]], [[1s9f|1s9f]], [[2au0|2au0]], [[2c28|2c28]], [[2v9w|2v9w]], [[2c2e|2c2e]], [[1ryr|1ryr]], [[2c2d|2c2d]], [[2uvv|2uvv]], [[1s10|1s10]], [[2br0|2br0]], [[2j6s|2j6s]], [[2uvr|2uvr]], [[2va3|2va3]], [[2v4q|2v4q]], [[2v4s|2v4s]], [[2v4t|2v4t]]</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2v4r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v4r OCA], [http://pdbe.org/2v4r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2v4r RCSB], [http://www.ebi.ac.uk/pdbsum/2v4r PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2v4r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v4r OCA], [http://pdbe.org/2v4r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2v4r RCSB], [http://www.ebi.ac.uk/pdbsum/2v4r PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2v4r ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 15: / Line 16: @@
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/v4/2v4r_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/v4/2v4r_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
@@ Line 46: / Line 47: @@
 [[Category: Stafford, J B]]
 [[Category: Szekely, J]]
+[[Category: Cytoplasm]]
 [[Category: Dna adduct]]
 [[Category: Dna damage]]

2v4r

From Proteopedia

Revision as of 08:08, 12 September 2018

Non-productive complex of the Y-family DNA polymerase Dpo4 with dGTP skipping the M1dG adduct to pair with the next template cytosine

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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