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1cxn

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Revision as of 08:26, 6 December 2017

REFINED THREE-DIMENSIONAL SOLUTION STRUCTURE OF A SNAKE CARDIOTOXIN: ANALYSIS OF THE SIDE-CHAIN ORGANISATION SUGGESTS THE EXISTENCE OF A POSSIBLE PHOSPHOLIPID BINDING SITE

Structural highlights

1cxn is a 1 chain structure with sequence from Black-necked spitting cobra. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	1cxo
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[CX1_NAJPA] Basic protein that bind to cell membrane and depolarizes cardiomyocytes. This cytotoxin also possesses lytic activity on many other cells, including red blood cells. Interaction with sulfatides in the cell membrane induces pore formation and cell internalization and is responsible for cytotoxicity in cardiomyocytes. It targets the mitochondrial membrane and induces mitochondrial swelling and fragmentation. Inhibits protein kinases C. It binds to the integrin alpha-V/beta-3 with a moderate affinity (By similarity).

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The solution structure of toxin gamma (60 residues, 4 disulfides) from Naja nigricollis was determined by proton nmr and molecular modeling with DIANA and X-PLOR. The structures were calculated using 489 distance and 81 dihedral angle constraints. The average atomic rms deviation between the nine refined structures and the average structure is 0.118 nm for the backbone atoms. Toxin gamma has an overall folding consisting of three loops stabilized by the four disulfides and forming a two- and a three-stranded beta-sheet (loop I and loops II, III, respectively). The same type of folding has been observed for two homologous cardiotoxins. The very close similarity of the solution structure of toxin gamma and the crystal structure of toxin VII4 includes details of the topological arrangement of numerous side chains. Among these are the conserved residues K12, K18, K35, and Y22, known to be critical for the cytolytic activity of toxin gamma. A cluster of hydrophobic side chains organized around Y22 is found on one side of the three-stranded beta-sheet and is spatially close to a group of three lysines (K12, K18, K35). The side chains of these lysines form a cationic site that can accommodate the binding of a phosphate ion as found in the crystal structure of toxin VII4. The hydrophobic cluster constitutes a possible binding site for the hydrophobic moiety of phospholipids. Together with the complementary cationic site, this hydrophobic surface can form a conserved site by which cardiotoxins bind to membrane phospholipids.

Refined three-dimensional solution structure of a snake cardiotoxin: analysis of the side-chain organization suggests the existence of a possible phospholipid binding site.,Gilquin B, Roumestand C, Zinn-Justin S, Menez A, Toma F Biopolymers. 1993 Nov;33(11):1659-75. PMID:8241426^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Gilquin B, Roumestand C, Zinn-Justin S, Menez A, Toma F. Refined three-dimensional solution structure of a snake cardiotoxin: analysis of the side-chain organization suggests the existence of a possible phospholipid binding site. Biopolymers. 1993 Nov;33(11):1659-75. PMID:8241426 doi:http://dx.doi.org/10.1002/bip.360331104

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1cxn"

@@ Line 1: / Line 1: @@
 ==REFINED THREE-DIMENSIONAL SOLUTION STRUCTURE OF A SNAKE CARDIOTOXIN: ANALYSIS OF THE SIDE-CHAIN ORGANISATION SUGGESTS THE EXISTENCE OF A POSSIBLE PHOSPHOLIPID BINDING SITE==
 <StructureSection load='1cxn' size='340' side='right' caption='[[1cxn]], [[NMR_Ensembles_of_Models | 1 NMR models]]' scene=''>
@@ Line 4: / Line 5: @@
 <table><tr><td colspan='2'>[[1cxn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Black-necked_spitting_cobra Black-necked spitting cobra]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CXN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CXN FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1cxo|1cxo]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cxn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cxn OCA], [http://pdbe.org/1cxn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1cxn RCSB], [http://www.ebi.ac.uk/pdbsum/1cxn PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cxn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cxn OCA], [http://pdbe.org/1cxn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1cxn RCSB], [http://www.ebi.ac.uk/pdbsum/1cxn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1cxn ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 12: / Line 13: @@
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cx/1cxn_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cx/1cxn_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
@@ Line 27: / Line 28: @@
 </div>
 <div class="pdbe-citations 1cxn" style="background-color:#fffaf0;"></div>
-==See Also==
-*[[Cardiotoxin|Cardiotoxin]]
 == References ==
 <references/>

1cxn

From Proteopedia

Revision as of 08:26, 6 December 2017

REFINED THREE-DIMENSIONAL SOLUTION STRUCTURE OF A SNAKE CARDIOTOXIN: ANALYSIS OF THE SIDE-CHAIN ORGANISATION SUGGESTS THE EXISTENCE OF A POSSIBLE PHOSPHOLIPID BINDING SITE

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

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