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2ww8

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Revision as of 09:09, 3 October 2018

Structure of the pilus adhesin (RrgA) from Streptococcus pneumoniae

Structural highlights

2ww8 is a 1 chain structure with sequence from "diplococcus_pneumoniae"_(klein_1884)_weichselbaum_1886 "diplococcus pneumoniae" (klein 1884) weichselbaum 1886. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Pili are fibrous virulence factors associated directly to the bacterial surface that play critical roles in adhesion and recognition of host cell receptors. The human pathogen Streptococcus pneumoniae carries a single pilus-related adhesin (RrgA) that is key for infection establishment and provides protection from bacterial challenge in animal infection models, but details of these roles remain unclear. Here we report the high-resolution crystal structure of RrgA, a 893-residue elongated macromolecule whose fold contains four domains presenting both eukaryotic and prokaryotic origins. RrgA harbors an integrin I collagen-recognition domain decorated with two inserted "arms" that fold into a positively charged cradle, as well as three "stalk-forming" domains. We show by site-specific mutagenesis, mass spectrometry, and thermal shift assays that intradomain isopeptide bonds play key roles in stabilizing RrgA's stalk. The high sequence similarity between RrgA and its homologs in other Gram-positive microorganisms suggests common strategies for ECM recognition and immune evasion.

Structural basis of host cell recognition by the pilus adhesin from Streptococcus pneumoniae.,Izore T, Contreras-Martel C, El Mortaji L, Manzano C, Terrasse R, Vernet T, Di Guilmi AM, Dessen A Structure. 2010 Jan 13;18(1):106-15. PMID:20152157^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Izore T, Contreras-Martel C, El Mortaji L, Manzano C, Terrasse R, Vernet T, Di Guilmi AM, Dessen A. Structural basis of host cell recognition by the pilus adhesin from Streptococcus pneumoniae. Structure. 2010 Jan 13;18(1):106-15. PMID:20152157 doi:10.1016/j.str.2009.10.019

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2ww8"

@@ Line 1: / Line 1: @@
 ==Structure of the pilus adhesin (RrgA) from Streptococcus pneumoniae==
 <StructureSection load='2ww8' size='340' side='right' caption='[[2ww8]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
@@ Line 4: / Line 5: @@
 <table><tr><td colspan='2'>[[2ww8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"diplococcus_pneumoniae"_(klein_1884)_weichselbaum_1886 "diplococcus pneumoniae" (klein 1884) weichselbaum 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WW8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WW8 FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ww8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ww8 OCA], [http://pdbe.org/2ww8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ww8 RCSB], [http://www.ebi.ac.uk/pdbsum/2ww8 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ww8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ww8 OCA], [http://pdbe.org/2ww8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ww8 RCSB], [http://www.ebi.ac.uk/pdbsum/2ww8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ww8 ProSAT]</span></td></tr>
 </table>
 == Evolutionary Conservation ==
@@ Line 10: / Line 11: @@
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ww/2ww8_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ww/2ww8_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>

2ww8

From Proteopedia

Revision as of 09:09, 3 October 2018

Structure of the pilus adhesin (RrgA) from Streptococcus pneumoniae

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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