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2uwe

From Proteopedia

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Revision as of 08:17, 12 September 2018

Large CDR3a loop alteration as a function of MHC mutation

Structural highlights

2uwe is a 10 chain structure with sequence from Human and Lk3 transgenic mice. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	1akj, 1ao7, 1aqd, 1b0g, 1b0r, 1bd2, 1duy, 1duz, 1eey, 1eez, 1hhg, 1hhh, 1hhi, 1hhj, 1hhk, 1hla, 1i1f, 1i1y, 1i4f, 1i7r, 1i7t, 1i7u, 1im3, 1jf1, 1jht, 1lp9, 1oga, 1p7q, 1qew, 1qr1, 1qrn, 1qse, 1qsf, 1s8d, 1s9w, 1s9x, 1s9y, 1t1w, 1t1x, 1t1y, 1t1z, 1t20, 1t21, 1t22, 1tvb, 1tvh, 1ur7, 2av1, 2av7, 2bnq, 2bnr, 2bsu, 2bsv, 2c7u, 2clr, 2gj6, 2jcc, 3hla, 1a1m, 1a1n, 1a1o, 1a6z, 1a9b, 1a9e, 1agb, 1agc, 1agd, 1age, 1agf, 1c16, 1ce6, 1cg9, 1de4, 1e27, 1e28, 1efx, 1exu, 1gzp, 1gzq, 1hsa, 1hsb, 1im9, 1jgd, 1jge, 1jnj, 1k5n, 1kpr, 1ktl, 1lds, 1m05, 1m6o, 1mhe, 1mi5, 1n2r, 1of2, 1ogt, 1onq, 1py4, 1q94, 1qlf, 1qqd, 1qvo, 1r3h, 1sys, 1syv, 1tmc, 1uqs, 1uxs, 1uxw, 1vgk, 1w0v, 1w0w, 1w72, 1x7q, 1xh3, 1xr8, 1xr9, 1xz0, 1ydp, 1ypz, 1zs8, 1zsd, 1zt4, 2a83, 2ak4, 2axf, 2axg, 2bck, 2bsr, 2bss, 2bst, 2bvq, 2cii, 2cik, 2d31, 2esv, 2f74, 2f8o, 2h26, 2hjk, 2hjl, 2hla
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[1A02_HUMAN] Involved in the presentation of foreign antigens to the immune system.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The keystone of the adaptive immune response is T cell receptor (TCR) recognition of peptide presented by major histocompatibility complex (pMHC) molecules. The crystal structure of AHIII TCR bound to MHC, HLA-A2, showed a large interface with an atypical binding orientation. MHC mutations in the interface of the proteins were tested for changes in TCR recognition. From the range of responses observed, three representative HLA-A2 mutants, T163A, W167A, and K66A, were selected for further study. Binding constants and co-crystal structures of the AHIII TCR and the three mutants were determined. K66 in HLA-A2 makes contacts with both peptide and TCR, and has been identified as a critical residue for recognition by numerous TCR. The K66A mutation resulted in the lowest AHIII T cell response and the lowest binding affinity, which suggests that the T cell response may correlate with affinity. Importantly, the K66A mutation does not affect the conformation of the peptide. The change in affinity appears to be due to a loss in hydrogen bonds in the interface as a result of a conformational change in the TCR complementarity-determining region 3 (CDR3) loop. Isothermal titration calorimetry confirmed the loss of hydrogen bonding by a large loss in enthalpy. Our findings are inconsistent with the notion that the CDR1 and CDR2 loops of the TCR are responsible for MHC restriction, while the CDR3 loops interact solely with the peptide. Instead, we present here an MHC mutation that does not change the conformation of the peptide, yet results in an altered conformation of a CDR3.

Single MHC mutation eliminates enthalpy associated with T cell receptor binding.,Miller PJ, Pazy Y, Conti B, Riddle D, Appella E, Collins EJ J Mol Biol. 2007 Oct 19;373(2):315-27. Epub 2007 Jul 26. PMID:17825839^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Miller PJ, Pazy Y, Conti B, Riddle D, Appella E, Collins EJ. Single MHC mutation eliminates enthalpy associated with T cell receptor binding. J Mol Biol. 2007 Oct 19;373(2):315-27. Epub 2007 Jul 26. PMID:17825839 doi:10.1016/j.jmb.2007.07.028

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2uwe"

@@ Line 1: / Line 1: @@
-==LARGE CDR3A LOOP ALTERATION AS A FUNCTION OF MHC MUTATION==
+==Large CDR3a loop alteration as a function of MHC mutation==
 <StructureSection load='2uwe' size='340' side='right' caption='[[2uwe]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
 == Structural highlights ==
 <table><tr><td colspan='2'>[[2uwe]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UWE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2UWE FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1akj|1akj]], [[1ao7|1ao7]], [[1aqd|1aqd]], [[1b0g|1b0g]], [[1b0r|1b0r]], [[1bd2|1bd2]], [[1duy|1duy]], [[1duz|1duz]], [[1eey|1eey]], [[1eez|1eez]], [[1hhg|1hhg]], [[1hhh|1hhh]], [[1hhi|1hhi]], [[1hhj|1hhj]], [[1hhk|1hhk]], [[1hla|1hla]], [[1i1f|1i1f]], [[1i1y|1i1y]], [[1i4f|1i4f]], [[1i7r|1i7r]], [[1i7t|1i7t]], [[1i7u|1i7u]], [[1im3|1im3]], [[1jf1|1jf1]], [[1jht|1jht]], [[1lp9|1lp9]], [[1oga|1oga]], [[1p7q|1p7q]], [[1qew|1qew]], [[1qr1|1qr1]], [[1qrn|1qrn]], [[1qse|1qse]], [[1qsf|1qsf]], [[1s8d|1s8d]], [[1s9w|1s9w]], [[1s9x|1s9x]], [[1s9y|1s9y]], [[1t1w|1t1w]], [[1t1x|1t1x]], [[1t1y|1t1y]], [[1t1z|1t1z]], [[1t20|1t20]], [[1t21|1t21]], [[1t22|1t22]], [[1tvb|1tvb]], [[1tvh|1tvh]], [[1ur7|1ur7]], [[2av1|2av1]], [[2av7|2av7]], [[2bnq|2bnq]], [[2bnr|2bnr]], [[2bsu|2bsu]], [[2bsv|2bsv]], [[2c7u|2c7u]], [[2clr|2clr]], [[2gj6|2gj6]], [[2jcc|2jcc]], [[3hla|3hla]], [[1a1m|1a1m]], [[1a1n|1a1n]], [[1a1o|1a1o]], [[1a6z|1a6z]], [[1a9b|1a9b]], [[1a9e|1a9e]], [[1agb|1agb]], [[1agc|1agc]], [[1agd|1agd]], [[1age|1age]], [[1agf|1agf]], [[1c16|1c16]], [[1ce6|1ce6]], [[1cg9|1cg9]], [[1de4|1de4]], [[1e27|1e27]], [[1e28|1e28]], [[1efx|1efx]], [[1exu|1exu]], [[1gzp|1gzp]], [[1gzq|1gzq]], [[1hsa|1hsa]], [[1hsb|1hsb]], [[1im9|1im9]], [[1jgd|1jgd]], [[1jge|1jge]], [[1jnj|1jnj]], [[1k5n|1k5n]], [[1kpr|1kpr]], [[1ktl|1ktl]], [[1lds|1lds]], [[1m05|1m05]], [[1m6o|1m6o]], [[1mhe|1mhe]], [[1mi5|1mi5]], [[1n2r|1n2r]], [[1of2|1of2]], [[1ogt|1ogt]], [[1onq|1onq]], [[1py4|1py4]], [[1q94|1q94]], [[1qlf|1qlf]], [[1qqd|1qqd]], [[1qvo|1qvo]], [[1r3h|1r3h]], [[1sys|1sys]], [[1syv|1syv]], [[1tmc|1tmc]], [[1uqs|1uqs]], [[1uxs|1uxs]], [[1uxw|1uxw]], [[1vgk|1vgk]], [[1w0v|1w0v]], [[1w0w|1w0w]], [[1w72|1w72]], [[1x7q|1x7q]], [[1xh3|1xh3]], [[1xr8|1xr8]], [[1xr9|1xr9]], [[1xz0|1xz0]], [[1ydp|1ydp]], [[1ypz|1ypz]], [[1zs8|1zs8]], [[1zsd|1zsd]], [[1zt4|1zt4]], [[2a83|2a83]], [[2ak4|2ak4]], [[2axf|2axf]], [[2axg|2axg]], [[2bck|2bck]], [[2bsr|2bsr]], [[2bss|2bss]], [[2bst|2bst]], [[2bvq|2bvq]], [[2cii|2cii]], [[2cik|2cik]], [[2d31|2d31]], [[2esv|2esv]], [[2f74|2f74]], [[2f8o|2f8o]], [[2h26|2h26]], [[2hjk|2hjk]], [[2hjl|2hjl]], [[2hla|2hla]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2uwe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uwe OCA], [http://pdbe.org/2uwe PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2uwe RCSB], [http://www.ebi.ac.uk/pdbsum/2uwe PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2uwe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uwe OCA], [http://pdbe.org/2uwe PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2uwe RCSB], [http://www.ebi.ac.uk/pdbsum/2uwe PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2uwe ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 12: / Line 13: @@
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/uw/2uwe_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/uw/2uwe_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>

2uwe

From Proteopedia

Revision as of 08:17, 12 September 2018

Large CDR3a loop alteration as a function of MHC mutation

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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