3f6p

From Proteopedia

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Revision as of 09:27, 25 October 2017

Crystal Structure of unphosphorelated receiver domain of YycF

Structural highlights

3f6p is a 1 chain structure with sequence from Bacsu. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	BSU40410, yycF (BACSU)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[YYCF_BACSU] Member of the two-component regulatory system YycG/YycF involved in the regulation of the ftsAZ operon, the yocH, ykvT, cwlO, lytE, ydjM, yjeA, yoeB genes and the tagAB and tagDEF operons. Probably phosphorylates YycF. Binds to the ftsAZ P1 promoter sequence in vitro. YycF has been shown to directly bind to the regulatory regions of yocH, ykvT, tagAB/tagDEF. Activates cwlO, lytE and ydjM and represses yoeB and yjeA.^[1] ^[2] ^[3]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

YycGF is a crucial signal transduction system for the regulation of cell-wall metabolism in low-G+C Gram-positive bacteria, which include many important human pathogens. The response regulator YycF receives signals from its cognate histidine kinase YycG through a phosphotransfer reaction and elicits responses through regulation of gene expression. The N-terminal regulatory domain of YycF from Bacillus subtilis was overproduced and purified. The protein was crystallized and X-ray data were collected to 1.95 A resolution with a completeness of 97.7% and an overall R(merge) of 7.7%. The crystals belonged to space group P3(1)21, with unit-cell parameters a = b = 59.50, c = 79.06 A.

Preliminary crystallographic studies of the regulatory domain of response regulator YycF from an essential two-component signal transduction system.,Zhao H, Heroux A, Sequeira RD, Tang L Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Jul 1;65(Pt, 7):719-22. Epub 2009 Jun 27. PMID:19574649^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Fabret C, Hoch JA. A two-component signal transduction system essential for growth of Bacillus subtilis: implications for anti-infective therapy. J Bacteriol. 1998 Dec;180(23):6375-83. PMID:9829949
↑ Fukuchi K, Kasahara Y, Asai K, Kobayashi K, Moriya S, Ogasawara N. The essential two-component regulatory system encoded by yycF and yycG modulates expression of the ftsAZ operon in Bacillus subtilis. Microbiology. 2000 Jul;146 ( Pt 7):1573-83. PMID:10878122
↑ Bisicchia P, Noone D, Lioliou E, Howell A, Quigley S, Jensen T, Jarmer H, Devine KM. The essential YycFG two-component system controls cell wall metabolism in Bacillus subtilis. Mol Microbiol. 2007 Jul;65(1):180-200. PMID:17581128 doi:http://dx.doi.org/MMI5782
↑ Zhao H, Heroux A, Sequeira RD, Tang L. Preliminary crystallographic studies of the regulatory domain of response regulator YycF from an essential two-component signal transduction system. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Jul 1;65(Pt, 7):719-22. Epub 2009 Jun 27. PMID:19574649 doi:10.1107/S1744309109022696

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3f6p"

@@ Line 1: / Line 1: @@
 ==Crystal Structure of unphosphorelated receiver domain of YycF==
 <StructureSection load='3f6p' size='340' side='right' caption='[[3f6p]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
@@ Line 4: / Line 5: @@
 <table><tr><td colspan='2'>[[3f6p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacsu Bacsu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3F6P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3F6P FirstGlance]. <br>
 </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BSU40410, yycF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 BACSU])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3f6p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3f6p OCA], [http://pdbe.org/3f6p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3f6p RCSB], [http://www.ebi.ac.uk/pdbsum/3f6p PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3f6p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3f6p OCA], [http://pdbe.org/3f6p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3f6p RCSB], [http://www.ebi.ac.uk/pdbsum/3f6p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3f6p ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 34: / Line 35: @@
 [[Category: Tang, L]]
 [[Category: Zhao, H]]
+[[Category: Cytoplasm]]
 [[Category: Dna binding protein]]
 [[Category: Dna-binding]]

3f6p

From Proteopedia

Revision as of 09:27, 25 October 2017

Crystal Structure of unphosphorelated receiver domain of YycF

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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