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1jjh

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Revision as of 09:22, 17 January 2018

E2 DNA-binding Domain from Bovine Papillomavirus Type 1

Structural highlights

1jjh is a 3 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	1by9, 2bop, 1f9f, 1jj4
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[VE2_BPV1] E2 regulates viral transcription and DNA replication. Binds to the E2RE response element (5'-ACCNNNNNNGGT-3') present in multiple copies in the regulatory region. Can either activate or repress transcription depending on E2RE's position with regards to proximal promoter elements. Repression occurs by sterically hindering the assembly of the transcription initiation complex. The E1-E2 complex binds to the origin of DNA replication.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The 2.5 A crystal structures of the DNA-binding domain of the E2 protein from bovine papillomavirus strain 1 and its complex with DNA are presented. E2 is a transcriptional regulatory protein that is also involved in viral DNA replication. It is the structural prototype for a novel class of DNA-binding proteins: dimeric beta-barrels with surface alpha-helices that serve as recognition helices. These helices contain the amino-acid residues involved in sequence-specifying interactions. The E2 proteins from different papillomavirus strains recognize and bind to the same consensus 12 base-pair DNA sequence. However, recent evidence from solution studies points to differences in the mechanisms by which E2 from the related viral strains bovine papillomavirus-1 and human papillomavirus-16 discriminate between DNA targets based on non-contacted nucleotide sequences. This report provides evidence that sequence-specific DNA-binding is accompanied by a rearrangement of protein subunits and deformation of the DNA. These results suggest that, along with DNA sequence-dependent conformational properties, protein subunit orientation plays a significant role in the mechanisms of target selection utilized by E2.

Subunit rearrangement accompanies sequence-specific DNA binding by the bovine papillomavirus-1 E2 protein.,Hegde RS, Wang AF, Kim SS, Schapira M J Mol Biol. 1998 Mar 6;276(4):797-808. PMID:9500927^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Hegde RS, Wang AF, Kim SS, Schapira M. Subunit rearrangement accompanies sequence-specific DNA binding by the bovine papillomavirus-1 E2 protein. J Mol Biol. 1998 Mar 6;276(4):797-808. PMID:9500927 doi:10.1006/jmbi.1997.1587

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1jjh"

@@ Line 1: / Line 1: @@
 ==E2 DNA-binding Domain from Bovine Papillomavirus Type 1==
 <StructureSection load='1jjh' size='340' side='right' caption='[[1jjh]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1jjh]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpv-1 Bpv-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JJH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1JJH FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1jjh]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JJH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1JJH FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1by9|1by9]], [[2bop|2bop]], [[1f9f|1f9f]], [[1jj4|1jj4]]</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">E2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10559 BPV-1])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jjh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jjh OCA], [http://pdbe.org/1jjh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1jjh RCSB], [http://www.ebi.ac.uk/pdbsum/1jjh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1jjh ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jjh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jjh OCA], [http://pdbe.org/1jjh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1jjh RCSB], [http://www.ebi.ac.uk/pdbsum/1jjh PDBsum]</span></td></tr>
 </table>
 == Function ==
@@ Line 13: / Line 13: @@
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jj/1jjh_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jj/1jjh_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
@@ Line 32: / Line 32: @@
 __TOC__
 </StructureSection>
-[[Category: Bpv-1]]
 [[Category: Hegde, R S]]
 [[Category: Kim, S S]]
 [[Category: Schapira, M]]
 [[Category: Wang, A F]]
+[[Category: Bpv-1]]
 [[Category: Dna binding protein]]
 [[Category: Dna-binding domain]]
 [[Category: E2]]

1jjh

From Proteopedia

Revision as of 09:22, 17 January 2018

E2 DNA-binding Domain from Bovine Papillomavirus Type 1

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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