1egk
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1egk.gif|left|200px]] | [[Image:1egk.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1egk", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | | | + | or leave the SCENE parameter empty for the default display. |
| - | | | + | --> |
| - | + | {{STRUCTURE_1egk| PDB=1egk | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''CRYSTAL STRUCTURE OF A NUCLEIC ACID FOUR-WAY JUNCTION''' | '''CRYSTAL STRUCTURE OF A NUCLEIC ACID FOUR-WAY JUNCTION''' | ||
| Line 19: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EGK OCA]. | |
==Reference== | ==Reference== | ||
Alternative conformations of a nucleic acid four-way junction., Nowakowski J, Shim PJ, Stout CD, Joyce GF, J Mol Biol. 2000 Jun 30;300(1):93-102. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10864501 10864501] | Alternative conformations of a nucleic acid four-way junction., Nowakowski J, Shim PJ, Stout CD, Joyce GF, J Mol Biol. 2000 Jun 30;300(1):93-102. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10864501 10864501] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Joyce, G F.]] | [[Category: Joyce, G F.]] | ||
[[Category: Nowakowski, J.]] | [[Category: Nowakowski, J.]] | ||
| Line 29: | Line 25: | ||
[[Category: Stout, C D.]] | [[Category: Stout, C D.]] | ||
[[Category: 10-23 dna enzyme]] | [[Category: 10-23 dna enzyme]] | ||
| - | [[Category: | + | [[Category: Four-way junction]] |
| - | [[Category: | + | [[Category: Nucleic acid]] |
| - | [[Category: | + | [[Category: Ribozyme]] |
| - | [[Category: | + | [[Category: Rna-dna complex]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:04:33 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 12:04, 2 May 2008
CRYSTAL STRUCTURE OF A NUCLEIC ACID FOUR-WAY JUNCTION
Overview
A crystal structure of a 108 nucleotide RNA-DNA complex containing a four-way junction was solved at 3.1 A resolution. The structure of the junction differs substantially from the "stacked-X" conformation observed previously, due to a 135 degrees rotation of the branches. Comparison of the two conformers provides insight into the factors contributing to the flexibility of four-way junctions. The stacked-X conformation maximizes base-stacking but causes unfavorable repulsion between phosphate groups, whereas the 135 degrees -rotated "crossed" conformation minimizes electrostatic clashes at the expense of reduced base-stacking. Despite the large rotation of the branches, both junction structures exhibit an antiparallel arrangement of the continuous strands and opposite polarity of the crossover strands.
About this Structure
Full crystallographic information is available from OCA.
Reference
Alternative conformations of a nucleic acid four-way junction., Nowakowski J, Shim PJ, Stout CD, Joyce GF, J Mol Biol. 2000 Jun 30;300(1):93-102. PMID:10864501 Page seeded by OCA on Fri May 2 15:04:33 2008
