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1xnx

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Revision as of 13:00, 12 October 2017

Crystal structure of constitutive androstane receptor

Structural highlights

1xnx is a 2 chain structure with sequence from Lk3 transgenic mice. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Gene:	Nr1i3, Car (LK3 transgenic mice)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[NR1I3_MOUSE] Binds and transactivates the retinoic acid response elements that control expression of the retinoic acid receptor beta 2 and alcohol dehydrogenase 3 genes. Transactivates both the phenobarbital responsive element module of the human CYP2B6 gene and the CYP3A4 xenobiotic response element (By similarity).^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The nuclear receptor CAR is a xenobiotic responsive transcription factor that plays a central role in the clearance of drugs and bilirubin while promoting cocaine and acetaminophen toxicity. In addition, CAR has established a "reverse" paradigm of nuclear receptor action where the receptor is active in the absence of ligand and inactive when bound to inverse agonists. We now report the crystal structure of murine CAR bound to the inverse agonist androstenol. Androstenol binds within the ligand binding pocket, but unlike many nuclear receptor ligands, it makes no contacts with helix H12/AF2. The transition from constitutive to basal activity (androstenol bound) appears to be associated with a ligand-induced kink between helices H10 and H11. This disrupts the previously predicted salt bridge that locks H12 in the transcriptionally active conformation. This mechanism of inverse agonism is distinct from traditional nuclear receptor antagonists thereby offering a new approach to receptor modulation.

Structure of the murine constitutive androstane receptor complexed to androstenol: a molecular basis for inverse agonism.,Shan L, Vincent J, Brunzelle JS, Dussault I, Lin M, Ianculescu I, Sherman MA, Forman BM, Fernandez EJ Mol Cell. 2004 Dec 22;16(6):907-17. PMID:15610734^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Waxman DJ. P450 gene induction by structurally diverse xenochemicals: central role of nuclear receptors CAR, PXR, and PPAR. Arch Biochem Biophys. 1999 Sep 1;369(1):11-23. PMID:10462436 doi:http://dx.doi.org/10.1006/abbi.1999.1351
↑ Shan L, Vincent J, Brunzelle JS, Dussault I, Lin M, Ianculescu I, Sherman MA, Forman BM, Fernandez EJ. Structure of the murine constitutive androstane receptor complexed to androstenol: a molecular basis for inverse agonism. Mol Cell. 2004 Dec 22;16(6):907-17. PMID:15610734 doi:10.1016/j.molcel.2004.11.037

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1xnx"

Categories: Lk3 transgenic mice | Fernandez, E | Crystal structure | Ligand receptor-transcription regulation complex | Nuclear receptor

@@ Line 1: / Line 1: @@
 ==Crystal structure of constitutive androstane receptor==
 <StructureSection load='1xnx' size='340' side='right' caption='[[1xnx]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATE:16,17-ANDROSTENE-3-OL'>ATE</scene></td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Nr1i3, Car ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xnx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xnx OCA], [http://pdbe.org/1xnx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1xnx RCSB], [http://www.ebi.ac.uk/pdbsum/1xnx PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xnx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xnx OCA], [http://pdbe.org/1xnx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1xnx RCSB], [http://www.ebi.ac.uk/pdbsum/1xnx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1xnx ProSAT]</span></td></tr>
 </table>
 == Function ==

1xnx

From Proteopedia

Revision as of 13:00, 12 October 2017

Crystal structure of constitutive androstane receptor

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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