1eht
From Proteopedia
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'''THEOPHYLLINE-BINDING RNA IN COMPLEX WITH THEOPHYLLINE, NMR, 10 STRUCTURES''' | '''THEOPHYLLINE-BINDING RNA IN COMPLEX WITH THEOPHYLLINE, NMR, 10 STRUCTURES''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EHT OCA]. | |
==Reference== | ==Reference== | ||
Interlocking structural motifs mediate molecular discrimination by a theophylline-binding RNA., Zimmermann GR, Jenison RD, Wick CL, Simorre JP, Pardi A, Nat Struct Biol. 1997 Aug;4(8):644-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9253414 9253414] | Interlocking structural motifs mediate molecular discrimination by a theophylline-binding RNA., Zimmermann GR, Jenison RD, Wick CL, Simorre JP, Pardi A, Nat Struct Biol. 1997 Aug;4(8):644-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9253414 9253414] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Pardi, A.]] | [[Category: Pardi, A.]] | ||
[[Category: Zimmermann, G R.]] | [[Category: Zimmermann, G R.]] | ||
| - | [[Category: | + | [[Category: Ribonucleic acid]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:07:01 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 12:07, 2 May 2008
THEOPHYLLINE-BINDING RNA IN COMPLEX WITH THEOPHYLLINE, NMR, 10 STRUCTURES
Overview
To visualize the interplay of RNA structural interactions in a ligand binding site, we have determined the solution structure of a high affinity RNA-theophylline complex using NMR spectroscopy. The structure provides insight into the ability of this in vitro selected RNA to discriminate theophylline from the structurally similar molecule caffeine. Numerous RNA structural motifs combine to form a well-ordered binding pocket where an intricate network of hydrogen bonds and stacking interactions lock the theophylline into the complex. Two internal loops interact to form the binding site which consists of a sandwich of three base triples. The complex also contains novel base-zipper and 1-3-2 stacking motifs, in addition to an adenosine platform and a reversed sugar. An important feature of the RNA is that many of the conserved core residues participate in multiple overlapping tertiary interactions. This complex illustrates how interlocking structural motifs can be assembled into a highly specific ligand-binding site that possesses high levels of affinity and molecular discrimination.
About this Structure
Full crystallographic information is available from OCA.
Reference
Interlocking structural motifs mediate molecular discrimination by a theophylline-binding RNA., Zimmermann GR, Jenison RD, Wick CL, Simorre JP, Pardi A, Nat Struct Biol. 1997 Aug;4(8):644-9. PMID:9253414 Page seeded by OCA on Fri May 2 15:07:01 2008
