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3bep

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Revision as of 08:01, 25 October 2017

Structure of a sliding clamp on DNA

Structural highlights

3bep is a 4 chain structure with sequence from "bacillus_coli"_migula_1895 "bacillus coli" migula 1895. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	2pol
Gene:	dnaN ("Bacillus coli" Migula 1895)
Activity:	DNA-directed DNA polymerase, with EC number 2.7.7.7
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[DPO3B_ECOLI] DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped onto DNA, it slides freely (bidirectional and ATP-independent) along duplex DNA.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The structure of the E. coli beta clamp polymerase processivity factor has been solved in complex with primed DNA. Interestingly, the clamp directly binds the DNA duplex and also forms a crystal contact with the ssDNA template strand, which binds into the protein-binding pocket of the clamp. We demonstrate that these clamp-DNA interactions function in clamp loading, perhaps by inducing the ring to close around DNA. Clamp binding to template ssDNA may also serve to hold the clamp at a primed site after loading or during switching of multiple factors on the clamp. Remarkably, the DNA is highly tilted as it passes through the beta ring. The pronounced 22 degrees angle of DNA through beta may enable DNA to switch between multiple factors bound to a single clamp simply by alternating from one protomer of the ring to the other.

Structure of a sliding clamp on DNA.,Georgescu RE, Kim SS, Yurieva O, Kuriyan J, Kong XP, O'Donnell M Cell. 2008 Jan 11;132(1):43-54. PMID:18191219^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Georgescu RE, Kim SS, Yurieva O, Kuriyan J, Kong XP, O'Donnell M. Structure of a sliding clamp on DNA. Cell. 2008 Jan 11;132(1):43-54. PMID:18191219 doi:10.1016/j.cell.2007.11.045

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3bep"

@@ Line 1: / Line 1: @@
 ==Structure of a sliding clamp on DNA==
 <StructureSection load='3bep' size='340' side='right' caption='[[3bep]], [[Resolution|resolution]] 1.92&Aring;' scene=''>
@@ Line 7: / Line 8: @@
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">dnaN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bep FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bep OCA], [http://pdbe.org/3bep PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3bep RCSB], [http://www.ebi.ac.uk/pdbsum/3bep PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bep FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bep OCA], [http://pdbe.org/3bep PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3bep RCSB], [http://www.ebi.ac.uk/pdbsum/3bep PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3bep ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 30: / Line 31: @@
 </div>
 <div class="pdbe-citations 3bep" style="background-color:#fffaf0;"></div>
-==See Also==
-*[[DNA polymerase|DNA polymerase]]
 == References ==
 <references/>

3bep

From Proteopedia

Revision as of 08:01, 25 October 2017

Structure of a sliding clamp on DNA

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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