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1z3h

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Revision as of 07:02, 25 April 2018

The exportin Cse1 in its cargo-free, cytoplasmic state

Structural highlights

1z3h is a 2 chain structure with sequence from Atcc 18824. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	1wa5
Gene:	Cse1 (ATCC 18824)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[CSE1_YEAST] Export receptor for importin alpha (SRP1). Mediates importin-alpha re-export from the nucleus to the cytoplasm after import substrates have been released into the nucleoplasm.^[1] ^[2] ^[3] ^[4]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Cse1 mediates nuclear export of importin alpha, the nuclear localization signal (NLS) import adaptor. We report the 3.1 A resolution structure of cargo-free Cse1, representing this HEAT repeat protein in its cytosolic state. Cse1 is compact, consisting of N- and C-terminal arches that interact to form a ring. Comparison with the structure of cargo-bound Cse1 shows a major conformational change leading to opening of the structure upon cargo binding. The largest structural changes occur within a hinge region centered at HEAT repeat 8. This repeat contains a conserved insertion that connects the RanGTP and importin alpha contact sites and that is essential for binding. In the cargo-free state, the RanGTP binding sites are occluded and the importin alpha sites are distorted. Mutations that destabilize the N- to C-terminal interaction uncouple importin alpha and Ran binding, suggesting that the closed conformation prevents association with importin alpha.

The structure of the nuclear export receptor Cse1 in its cytosolic state reveals a closed conformation incompatible with cargo binding.,Cook A, Fernandez E, Lindner D, Ebert J, Schlenstedt G, Conti E Mol Cell. 2005 Apr 29;18(3):355-67. PMID:15866177^[5]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kunzler M, Hurt EC. Cse1p functions as the nuclear export receptor for importin alpha in yeast. FEBS Lett. 1998 Aug 21;433(3):185-90. PMID:9744791
↑ Hood JK, Silver PA. Cse1p is required for export of Srp1p/importin-alpha from the nucleus in Saccharomyces cerevisiae. J Biol Chem. 1998 Dec 25;273(52):35142-6. PMID:9857050
↑ Solsbacher J, Maurer P, Bischoff FR, Schlenstedt G. Cse1p is involved in export of yeast importin alpha from the nucleus. Mol Cell Biol. 1998 Nov;18(11):6805-15. PMID:9774694
↑ Schroeder AJ, Chen XH, Xiao Z, Fitzgerald-Hayes M. Genetic evidence for interactions between yeast importin alpha (Srp1p) and its nuclear export receptor, Cse1p. Mol Gen Genet. 1999 Jun;261(4-5):788-95. PMID:10394916
↑ Cook A, Fernandez E, Lindner D, Ebert J, Schlenstedt G, Conti E. The structure of the nuclear export receptor Cse1 in its cytosolic state reveals a closed conformation incompatible with cargo binding. Mol Cell. 2005 Apr 29;18(3):355-67. PMID:15866177 doi:10.1016/j.molcel.2005.03.021

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1z3h"

@@ Line 1: / Line 1: @@
 ==The exportin Cse1 in its cargo-free, cytoplasmic state==
 <StructureSection load='1z3h' size='340' side='right' caption='[[1z3h]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
@@ Line 6: / Line 7: @@
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1wa5|1wa5]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Cse1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1z3h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z3h OCA], [http://pdbe.org/1z3h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1z3h RCSB], [http://www.ebi.ac.uk/pdbsum/1z3h PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1z3h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z3h OCA], [http://pdbe.org/1z3h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1z3h RCSB], [http://www.ebi.ac.uk/pdbsum/1z3h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1z3h ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 14: / Line 15: @@
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z3/1z3h_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z3/1z3h_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>

1z3h

From Proteopedia

Revision as of 07:02, 25 April 2018

The exportin Cse1 in its cargo-free, cytoplasmic state

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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