1esg

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[[Image:1esg.gif|left|200px]]
[[Image:1esg.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1esg |SIZE=350|CAPTION= <scene name='initialview01'>1esg</scene>, resolution 1.90&Aring;
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The line below this paragraph, containing "STRUCTURE_1esg", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1esg| PDB=1esg | SCENE= }}
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|RELATEDENTRY=[[1bam|1BAM]], [[2bam|2BAM]], [[3bam|3BAM]], [[1bhm|1BHM]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1esg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1esg OCA], [http://www.ebi.ac.uk/pdbsum/1esg PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1esg RCSB]</span>
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}}
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'''RESTRICTION ENDONUCLEASE BAMHI BOUND TO A NON-SPECIFIC DNA.'''
'''RESTRICTION ENDONUCLEASE BAMHI BOUND TO A NON-SPECIFIC DNA.'''
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[[Category: Aggarwal, A K.]]
[[Category: Aggarwal, A K.]]
[[Category: Viadiu, H.]]
[[Category: Viadiu, H.]]
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[[Category: non-specific dna-protein complex.]]
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[[Category: Non-specific dna-protein complex.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:28:03 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:08:41 2008''
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Revision as of 12:28, 2 May 2008

Image:1esg.gif

Template:STRUCTURE 1esg

RESTRICTION ENDONUCLEASE BAMHI BOUND TO A NON-SPECIFIC DNA.


Overview

The central problem faced by DNA binding proteins is how to select the correct DNA sequence from the sea of nonspecific sequences in a cell. The problem is particularly acute for bacterial restriction enzymes because cleavage at an incorrect DNA site could be lethal. To understand the basis of this selectivity, we report here the crystal structure of endonuclease BamHI bound to noncognate DNA. We show that, despite only a single base pair change in the recognition sequence, the enzyme adopts an open configuration that is on the pathway between free and specifically bound forms of the enzyme. Surprisingly, the DNA drops out of the binding cleft with a total loss of base-specific and backbone contacts. Taken together, the structure provides a remarkable snapshot of an enzyme poised for linear diffusion (rather than cleavage) along the DNA.

About this Structure

1ESG is a Single protein structure of sequence from Bacillus amyloliquefaciens. Full crystallographic information is available from OCA.

Reference

Structure of BamHI bound to nonspecific DNA: a model for DNA sliding., Viadiu H, Aggarwal AK, Mol Cell. 2000 May;5(5):889-95. PMID:10882125 Page seeded by OCA on Fri May 2 15:28:03 2008

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