1ewj

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[[Image:1ewj.gif|left|200px]]
[[Image:1ewj.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1ewj |SIZE=350|CAPTION= <scene name='initialview01'>1ewj</scene>, resolution 2.50&Aring;
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The line below this paragraph, containing "STRUCTURE_1ewj", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=BLM:BLEOMYCIN+A2'>BLM</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE= TRANSPOSON TN5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573 Klebsiella pneumoniae])
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-->
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|DOMAIN=
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{{STRUCTURE_1ewj| PDB=1ewj | SCENE= }}
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|RELATEDENTRY=[[1qto|1QTO]], [[1byl|1BYL]], [[1ecs|1ECS]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ewj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ewj OCA], [http://www.ebi.ac.uk/pdbsum/1ewj PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ewj RCSB]</span>
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}}
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'''CRYSTAL STRUCTURE OF BLEOMYCIN-BINDING PROTEIN COMPLEXED WITH BLEOMYCIN'''
'''CRYSTAL STRUCTURE OF BLEOMYCIN-BINDING PROTEIN COMPLEXED WITH BLEOMYCIN'''
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[[Category: Matoba, Y.]]
[[Category: Matoba, Y.]]
[[Category: Sugiyama, M.]]
[[Category: Sugiyama, M.]]
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[[Category: antibiotics resistance]]
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[[Category: Antibiotics resistance]]
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[[Category: bleomycin]]
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[[Category: Bleomycin]]
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[[Category: bleomycin-binding protein]]
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[[Category: Bleomycin-binding protein]]
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[[Category: homodimer]]
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[[Category: Homodimer]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:36:05 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:10:58 2008''
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Revision as of 12:36, 2 May 2008

Image:1ewj.gif

Template:STRUCTURE 1ewj

CRYSTAL STRUCTURE OF BLEOMYCIN-BINDING PROTEIN COMPLEXED WITH BLEOMYCIN


Overview

The transposon Tn5 carries a gene designated ble that confers resistance to bleomycin (Bm). In this study, we determined the x-ray crystal structures of the ble gene product, designated BLMT, uncomplexed and complexed with Bm at 1.7 and 2.5 A resolution, respectively. The structure of BLMT is a dimer with two Bm-binding pockets composed of two large concavities and two long grooves. This crystal structure of BLMT complexed with Bm gives a precise mode for binding of the antibiotic to BLMT. The conformational change of BLMT generated by binding to Bm occurs at a beta-turn composed of the residues from Gln(97) to Thr(102). Crystallographic analysis of Bm bound to BLMT shows that two thiazolium rings of the bithiazole moiety are in the trans conformation. The axial ligand, which binds a metal ion, seems to be the primary amine in the beta-aminoalanine moiety. This report, which is the first with regard to the x-ray crystal structure of Bm, shows that the bithiazole moiety of Bm is far from the metal-binding domain. That is, Bm complexed with BLMT takes a more extended form than the drug complexed with DNA.

About this Structure

1EWJ is a Single protein structure of sequence from Klebsiella pneumoniae. Full crystallographic information is available from OCA.

Reference

Crystal structures of the transposon Tn5-carried bleomycin resistance determinant uncomplexed and complexed with bleomycin., Maruyama M, Kumagai T, Matoba Y, Hayashida M, Fujii T, Hata Y, Sugiyama M, J Biol Chem. 2001 Mar 30;276(13):9992-9. Epub 2000 Dec 29. PMID:11134052 Page seeded by OCA on Fri May 2 15:36:05 2008

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