1f3y
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1f3y.jpg|left|200px]] | [[Image:1f3y.jpg|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1f3y", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | | | + | --> |
| - | | | + | {{STRUCTURE_1f3y| PDB=1f3y | SCENE= }} |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''SOLUTION STRUCTURE OF THE NUDIX ENZYME DIADENOSINE TETRAPHOSPHATE HYDROLASE FROM LUPINUS ANGUSTIFOLIUS L.''' | '''SOLUTION STRUCTURE OF THE NUDIX ENZYME DIADENOSINE TETRAPHOSPHATE HYDROLASE FROM LUPINUS ANGUSTIFOLIUS L.''' | ||
| Line 23: | Line 20: | ||
==Reference== | ==Reference== | ||
The three-dimensional structure of the Nudix enzyme diadenosine tetraphosphate hydrolase from Lupinus angustifolius L., Swarbrick JD, Bashtannyk T, Maksel D, Zhang XR, Blackburn GM, Gayler KR, Gooley PR, J Mol Biol. 2000 Oct 6;302(5):1165-77. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11183782 11183782] | The three-dimensional structure of the Nudix enzyme diadenosine tetraphosphate hydrolase from Lupinus angustifolius L., Swarbrick JD, Bashtannyk T, Maksel D, Zhang XR, Blackburn GM, Gayler KR, Gooley PR, J Mol Biol. 2000 Oct 6;302(5):1165-77. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11183782 11183782] | ||
| - | [[Category: Bis(5'-nucleosyl)-tetraphosphatase (asymmetrical)]] | ||
[[Category: Lupinus angustifolius]] | [[Category: Lupinus angustifolius]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 34: | Line 30: | ||
[[Category: Zhang, X R.]] | [[Category: Zhang, X R.]] | ||
[[Category: 2-stranded antiparallel sheet]] | [[Category: 2-stranded antiparallel sheet]] | ||
| - | [[Category: | + | [[Category: Alpha-beta-alpha sandwich]] |
| - | [[Category: | + | [[Category: Enzyme,mixed 4-stranded beta sheet]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:52:02 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 12:52, 2 May 2008
SOLUTION STRUCTURE OF THE NUDIX ENZYME DIADENOSINE TETRAPHOSPHATE HYDROLASE FROM LUPINUS ANGUSTIFOLIUS L.
Overview
The solution structure of diadenosine 5',5-P1,P4-tetraphosphate hydrolase from Lupinus angustifolius L., an enzyme of the Nudix family, has been determined by heteronuclear NMR, using a torsion angle dynamics/simulated annealing protocol based on approximately 12 interresidue NOEs per residue. The structure represents the first Ap4A hydrolase to be determined, and sequence homology suggests that other members will have the same fold. The family of structures shows a well-defined fold comprised of a central four-stranded mixed beta-sheet, a two-stranded antiparallel beta-sheet and three helices (alphaI, alphaIII, alphaIV). The root-mean-squared deviation for the backbone (C',O,N,Calpha) of the rigid parts (residues 9 to 75, 97 to 115, 125 to 160) of the protein is 0.32 A. Several regions, however, show lower definition, particularly an isolated helix (alphaII) that connects two strands of the central sheet. This poor definition is mainly due to a lack of long-range NOEs between alphaII and other parts of the protein. Mapping conserved residues outside of the Nudix signature and those sensitive to an Ap4A analogue suggests that the adenosine-ribose moiety of the substrate binds into a large cleft above the four-stranded beta-sheet. Four conserved glutamate residues (Glu55, Glu58, Glu59 and Glu125) form a cluster that most likely ligates an essential magnesium ion, however, Gly41 also an expected magnesium ligand, is distant from this cluster.
About this Structure
1F3Y is a Single protein structure of sequence from Lupinus angustifolius. Full crystallographic information is available from OCA.
Reference
The three-dimensional structure of the Nudix enzyme diadenosine tetraphosphate hydrolase from Lupinus angustifolius L., Swarbrick JD, Bashtannyk T, Maksel D, Zhang XR, Blackburn GM, Gayler KR, Gooley PR, J Mol Biol. 2000 Oct 6;302(5):1165-77. PMID:11183782 Page seeded by OCA on Fri May 2 15:52:02 2008
