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1xcb

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Revision as of 07:03, 18 April 2018

X-ray Structure of a Rex-Family Repressor/NADH Complex from Thermus Aquaticus

Structural highlights

1xcb is a 7 chain structure with sequence from Atcc 25104. This structure supersedes the now removed PDB entry 1r72. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
NonStd Res:
Gene:	rex (ATCC 25104)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT, TOPSAN

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The redox-sensing repressor Rex regulates transcription of respiratory genes in response to the intra cellular NADH/NAD(+) redox poise. As a step toward elucidating the molecular mechanism of NADH/NAD(+) sensing, the X-ray structure of Thermus aquaticus Rex (T-Rex) bound to effector NADH has been determined at 2.9 A resolution. The fold of the C-terminal domain of T-Rex is characteristic of NAD(H)-dependent enzymes, whereas the N-terminal domain is similar to a winged helix DNA binding motif. T-Rex dimerization is primarily mediated by "domain-swapped" alpha helices. Each NADH molecule binds to the C-terminal domain near the dimer interface. In contrast to NAD(H)-dependent enzymes, the nicotinamide is deeply buried within a hydrophobic pocket that appears to preclude substrate entry. We show that T-Rex binds to the Rex operator, and NADH but not NAD(+) inhibits T-Rex/DNA binding activity. A mechanism for redox sensing by Rex family members is proposed by analogy with domain closure of NAD(H)-dependent enzymes.

X-ray structure of a Rex-family repressor/NADH complex insights into the mechanism of redox sensing.,Sickmier EA, Brekasis D, Paranawithana S, Bonanno JB, Paget MS, Burley SK, Kielkopf CL Structure. 2005 Jan;13(1):43-54. PMID:15642260^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Sickmier EA, Brekasis D, Paranawithana S, Bonanno JB, Paget MS, Burley SK, Kielkopf CL. X-ray structure of a Rex-family repressor/NADH complex insights into the mechanism of redox sensing. Structure. 2005 Jan;13(1):43-54. PMID:15642260 doi:http://dx.doi.org/10.1016/j.str.2004.10.012

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1xcb"

@@ Line 1: / Line 1: @@
 ==X-ray Structure of a Rex-Family Repressor/NADH Complex from Thermus Aquaticus==
 <StructureSection load='1xcb' size='340' side='right' caption='[[1xcb]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
@@ Line 6: / Line 7: @@
 <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rex ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=271 ATCC 25104])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xcb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xcb OCA], [http://pdbe.org/1xcb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1xcb RCSB], [http://www.ebi.ac.uk/pdbsum/1xcb PDBsum], [http://www.topsan.org/Proteins/NYSGXRC/1xcb TOPSAN]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xcb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xcb OCA], [http://pdbe.org/1xcb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1xcb RCSB], [http://www.ebi.ac.uk/pdbsum/1xcb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1xcb ProSAT], [http://www.topsan.org/Proteins/NYSGXRC/1xcb TOPSAN]</span></td></tr>
 </table>
 == Evolutionary Conservation ==
@@ Line 12: / Line 13: @@
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xc/1xcb_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xc/1xcb_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>

1xcb

From Proteopedia

Revision as of 07:03, 18 April 2018

X-ray Structure of a Rex-Family Repressor/NADH Complex from Thermus Aquaticus

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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