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3hyh

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Revision as of 07:51, 1 November 2017

Crystal structure of the protein kinase domain of yeast AMP-activated protein kinase Snf1

Structural highlights

3hyh is a 2 chain structure with sequence from Atcc 18824. This structure supersedes the now removed PDB entries 3fam and 2eue. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	CAT1, CCR1, D8035.20, GLC2, PAS14, SNF1, YDR477W (ATCC 18824)
Activity:	Non-specific serine/threonine protein kinase, with EC number 2.7.11.1
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[SNF1_YEAST] Essential for release from glucose repression. It interacts and has functional relationship to the regulatory protein SNF4. Could phosphorylate CAT8. Phosphorylates histone H3 to form H3S10ph, which promotes H3K14ac formation, and which is required for transcriptional activation through TBP recruitment to the promoters.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

AMP-activated protein kinase (AMPK) is a master metabolic regulator, and is an important target for drug development against diabetes, obesity, and other diseases. AMPK is a hetero-trimeric enzyme, with a catalytic (alpha) subunit, and two regulatory (beta and gamma) subunits. Here we report the crystal structure at 2.2A resolution of the protein kinase domain (KD) of the catalytic subunit of yeast AMPK (commonly known as SNF1). The Snf1-KD structure shares strong similarity to other protein kinases, with a small N-terminal lobe and a large C-terminal lobe. Two negative surface patches in the structure may be important for the recognition of the substrates of this kinase.

Crystal structure of the protein kinase domain of yeast AMP-activated protein kinase Snf1.,Rudolph MJ, Amodeo GA, Bai Y, Tong L Biochem Biophys Res Commun. 2005 Dec 2;337(4):1224-8. Epub 2005 Oct 7. PMID:16236260^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Lo WS, Gamache ER, Henry KW, Yang D, Pillus L, Berger SL. Histone H3 phosphorylation can promote TBP recruitment through distinct promoter-specific mechanisms. EMBO J. 2005 Mar 9;24(5):997-1008. Epub 2005 Feb 17. PMID:15719021 doi:7600577
↑ Rudolph MJ, Amodeo GA, Bai Y, Tong L. Crystal structure of the protein kinase domain of yeast AMP-activated protein kinase Snf1. Biochem Biophys Res Commun. 2005 Dec 2;337(4):1224-8. Epub 2005 Oct 7. PMID:16236260 doi:10.1016/j.bbrc.2005.09.181

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3hyh"

@@ Line 1: / Line 1: @@
 ==Crystal structure of the protein kinase domain of yeast AMP-activated protein kinase Snf1==
 <StructureSection load='3hyh' size='340' side='right' caption='[[3hyh]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CAT1, CCR1, D8035.20, GLC2, PAS14, SNF1, YDR477W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hyh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hyh OCA], [http://pdbe.org/3hyh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3hyh RCSB], [http://www.ebi.ac.uk/pdbsum/3hyh PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hyh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hyh OCA], [http://pdbe.org/3hyh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3hyh RCSB], [http://www.ebi.ac.uk/pdbsum/3hyh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3hyh ProSAT]</span></td></tr>
 </table>
 == Function ==

3hyh

From Proteopedia

Revision as of 07:51, 1 November 2017

Crystal structure of the protein kinase domain of yeast AMP-activated protein kinase Snf1

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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