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2jks

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Revision as of 07:22, 16 November 2017

Crystal structure of the the bradyzoite specific antigen BSR4 from toxoplasma gondii.

Structural highlights

2jks is a 1 chain structure with sequence from Toxgm. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Toxoplasma gondii is an obligate intracellular protozoan parasite that infects nearly one-third of the human population. The success of T. gondii is based on its complex life cycle; a lytic tachyzoite form disseminates infection, whereas an encysted bradyzoite form establishes a latent, chronic infection. Persistence and transmissibility is central to the survival of the parasite and is, in part, mediated by a family of antigenically distinct surface antigen glycoprotein (SAG)-related sequences (SRS) adhesins that play a dual role in host cell attachment and host immune evasion. More than 160 members of the SRS family have been identified with only the tachyzoite-expressed SAG1 structurally characterized. Here we report the first structural description of the bradyzoite adhesin BSR4 using x-ray crystallography and small angle x-ray scattering. The 1.90-A crystal structure of BSR4 reveals an architecture comprised of tandem beta sandwich domains organized in a head to tail fashion with the N-terminal domain responsible for dimer formation. A restructured topology in BSR4 results in a ligand-binding site that is significantly reorganized in both structure and chemistry relative to SAG1, consistent with BSR4 binding a distinct physiological ligand. The small angle x-ray scattering solution structure of BSR4 highlights a potentially important structural role for the interdomain polymorphic linker that imparts significant flexibility that may promote structural adaptation during ligand binding. This study reveals an unexpected level of structural diversity within the SRS superfamily and provides important insight into the role of these virulence factors.

Structural characterization of the bradyzoite surface antigen (BSR4) from Toxoplasma gondii, a unique addition to the surface antigen glycoprotein 1-related superfamily.,Crawford J, Grujic O, Bruic E, Czjzek M, Grigg ME, Boulanger MJ J Biol Chem. 2009 Apr 3;284(14):9192-8. Epub 2009 Jan 20. PMID:19155215^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Crawford J, Grujic O, Bruic E, Czjzek M, Grigg ME, Boulanger MJ. Structural characterization of the bradyzoite surface antigen (BSR4) from Toxoplasma gondii, a unique addition to the surface antigen glycoprotein 1-related superfamily. J Biol Chem. 2009 Apr 3;284(14):9192-8. Epub 2009 Jan 20. PMID:19155215 doi:10.1074/jbc.M808714200

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2jks"

@@ Line 1: / Line 1: @@
-==CRYSTAL STRUCTURE OF THE THE BRADYZOITE SPECIFIC ANTIGEN BSR4 FROM TOXOPLASMA GONDII.==
+==Crystal structure of the the bradyzoite specific antigen BSR4 from toxoplasma gondii.==
 <StructureSection load='2jks' size='340' side='right' caption='[[2jks]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2jks]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Toxoplasma_gondii_me49 Toxoplasma gondii me49]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JKS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JKS FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2jks]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Toxgm Toxgm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JKS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JKS FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jks OCA], [http://pdbe.org/2jks PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2jks RCSB], [http://www.ebi.ac.uk/pdbsum/2jks PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jks OCA], [http://pdbe.org/2jks PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2jks RCSB], [http://www.ebi.ac.uk/pdbsum/2jks PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2jks ProSAT]</span></td></tr>
 </table>
 == Evolutionary Conservation ==
@@ Line 29: / Line 30: @@
 __TOC__
 </StructureSection>
-[[Category: Toxoplasma gondii me49]]
+[[Category: Toxgm]]
 [[Category: Boulanger, M J]]
 [[Category: Bruic, E]]

2jks

From Proteopedia

Revision as of 07:22, 16 November 2017

Crystal structure of the the bradyzoite specific antigen BSR4 from toxoplasma gondii.

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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