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3hzq

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Revision as of 07:53, 1 November 2017

Structure of a tetrameric MscL in an expanded intermediate state

Structural highlights

3hzq is a 1 chain structure with sequence from Staaw. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	mscL (STAAW)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[MSCL_STAAW] Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The ability of cells to sense and respond to mechanical force underlies diverse processes such as touch and hearing in animals, gravitropism in plants, and bacterial osmoregulation. In bacteria, mechanosensation is mediated by the mechanosensitive channels of large (MscL), small (MscS), potassium-dependent (MscK) and mini (MscM) conductances. These channels act as 'emergency relief valves' protecting bacteria from lysis upon acute osmotic down-shock. Among them, MscL has been intensively studied since the original identification and characterization 15 years ago. MscL is reversibly and directly gated by changes in membrane tension. In the open state, MscL forms a non-selective 3 nS conductance channel which gates at tensions close to the lytic limit of the bacterial membrane. An earlier crystal structure at 3.5 A resolution of a pentameric MscL from Mycobacterium tuberculosis represents a closed-state or non-conducting conformation. MscL has a complex gating behaviour; it exhibits several intermediates between the closed and open states, including one putative non-conductive expanded state and at least three sub-conducting states. Although our understanding of the closed and open states of MscL has been increasing, little is known about the structures of the intermediate states despite their importance in elucidating the complete gating process of MscL. Here we present the crystal structure of a carboxy-terminal truncation mutant (Delta95-120) of MscL from Staphylococcus aureus (SaMscL(CDelta26)) at 3.8 A resolution. Notably, SaMscL(CDelta26) forms a tetrameric channel with both transmembrane helices tilted away from the membrane normal at angles close to that inferred for the open state, probably corresponding to a non-conductive but partially expanded intermediate state.

Structure of a tetrameric MscL in an expanded intermediate state.,Liu Z, Gandhi CS, Rees DC Nature. 2009 Sep 3;461(7260):120-4. Epub 2009 Aug 23. PMID:19701184^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Liu Z, Gandhi CS, Rees DC. Structure of a tetrameric MscL in an expanded intermediate state. Nature. 2009 Sep 3;461(7260):120-4. Epub 2009 Aug 23. PMID:19701184 doi:10.1038/nature08277

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3hzq"

@@ Line 1: / Line 1: @@
 ==Structure of a tetrameric MscL in an expanded intermediate state==
 <StructureSection load='3hzq' size='340' side='right' caption='[[3hzq]], [[Resolution|resolution]] 3.82&Aring;' scene=''>
@@ Line 4: / Line 5: @@
 <table><tr><td colspan='2'>[[3hzq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Staaw Staaw]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HZQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HZQ FirstGlance]. <br>
 </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mscL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=196620 STAAW])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hzq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hzq OCA], [http://pdbe.org/3hzq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3hzq RCSB], [http://www.ebi.ac.uk/pdbsum/3hzq PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hzq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hzq OCA], [http://pdbe.org/3hzq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3hzq RCSB], [http://www.ebi.ac.uk/pdbsum/3hzq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3hzq ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 27: / Line 28: @@
 </div>
 <div class="pdbe-citations 3hzq" style="background-color:#fffaf0;"></div>
-==See Also==
-*[[Ion channels|Ion channels]]
 == References ==
 <references/>

3hzq

From Proteopedia

Revision as of 07:53, 1 November 2017

Structure of a tetrameric MscL in an expanded intermediate state

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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