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1zzn

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Revision as of 11:33, 12 October 2017

Crystal structure of a group I intron/two exon complex that includes all catalytic metal ion ligands.

Structural highlights

1zzn is a 4 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
NonStd Res:	, ,
Related:	1u6b
Gene:	SNRPA (HUMAN)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[SNRPA_HUMAN] Binds stem loop II of U1 snRNA. It is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP. In a snRNP-free form (SF-A) may be involved in coupled pre-mRNA splicing and polyadenylation process. Binds preferentially to the 5'-UGCAC-3' motif in vitro.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

We report the 3.4 angstrom crystal structure of a catalytically active group I intron splicing intermediate containing the complete intron, both exons, the scissile phosphate, and all of the functional groups implicated in catalytic metal ion coordination, including the 2'-OH of the terminal guanosine. This structure suggests that, like protein phosphoryltransferases, an RNA phosphoryltransferase can use a two-metal-ion mechanism. Two Mg2+ ions are positioned 3.9 angstroms apart and are directly coordinated by all six of the biochemically predicted ligands. The evolutionary convergence of RNA and protein active sites on the same inorganic architecture highlights the intrinsic chemical capacity of the two-metal-ion catalytic mechanism for phosphoryl transfer.

Structural evidence for a two-metal-ion mechanism of group I intron splicing.,Stahley MR, Strobel SA Science. 2005 Sep 2;309(5740):1587-90. PMID:16141079^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Lutz CS, Cooke C, O'Connor JP, Kobayashi R, Alwine JC. The snRNP-free U1A (SF-A) complex(es): identification of the largest subunit as PSF, the polypyrimidine-tract binding protein-associated splicing factor. RNA. 1998 Dec;4(12):1493-9. PMID:9848648
↑ Stahley MR, Strobel SA. Structural evidence for a two-metal-ion mechanism of group I intron splicing. Science. 2005 Sep 2;309(5740):1587-90. PMID:16141079 doi:309/5740/1587

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1zzn"

@@ Line 1: / Line 1: @@
 ==Crystal structure of a group I intron/two exon complex that includes all catalytic metal ion ligands.==
 <StructureSection load='1zzn' size='340' side='right' caption='[[1zzn]], [[Resolution|resolution]] 3.37&Aring;' scene=''>
@@ Line 7: / Line 8: @@
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1u6b|1u6b]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SNRPA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zzn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zzn OCA], [http://pdbe.org/1zzn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1zzn RCSB], [http://www.ebi.ac.uk/pdbsum/1zzn PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zzn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zzn OCA], [http://pdbe.org/1zzn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1zzn RCSB], [http://www.ebi.ac.uk/pdbsum/1zzn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1zzn ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 32: / Line 33: @@
 ==See Also==
-*[[Group I intron|Group I intron]]
 *[[Kink-turn motif|Kink-turn motif]]
-*[[Nucleoprotein|Nucleoprotein]]
 *[[Ribozyme|Ribozyme]]
 *[[User:Wayne Decatur/kink-turn motif|User:Wayne Decatur/kink-turn motif]]

1zzn

From Proteopedia

Revision as of 11:33, 12 October 2017

Crystal structure of a group I intron/two exon complex that includes all catalytic metal ion ligands.

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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