1fof

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[[Image:1fof.gif|left|200px]]
[[Image:1fof.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1fof |SIZE=350|CAPTION= <scene name='initialview01'>1fof</scene>, resolution 2.00&Aring;
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The line below this paragraph, containing "STRUCTURE_1fof", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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|DOMAIN=
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{{STRUCTURE_1fof| PDB=1fof | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fof FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fof OCA], [http://www.ebi.ac.uk/pdbsum/1fof PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1fof RCSB]</span>
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}}
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'''CRYSTAL STRUCTURE OF THE CLASS D BETA-LACTAMASE OXA-10'''
'''CRYSTAL STRUCTURE OF THE CLASS D BETA-LACTAMASE OXA-10'''
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[[Category: Page, M G.P.]]
[[Category: Page, M G.P.]]
[[Category: Strynadka, N C.J.]]
[[Category: Strynadka, N C.J.]]
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[[Category: beta-lactamase]]
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[[Category: Beta-lactamase]]
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[[Category: class-d]]
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[[Category: Class-d]]
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[[Category: cobalt,]]
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[[Category: Cobalt]]
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[[Category: oxa-10]]
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[[Category: Oxa-10]]
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[[Category: oxacillinase]]
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[[Category: Oxacillinase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 16:34:26 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:26:58 2008''
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Revision as of 13:34, 2 May 2008

Image:1fof.gif

Template:STRUCTURE 1fof

CRYSTAL STRUCTURE OF THE CLASS D BETA-LACTAMASE OXA-10


Overview

We report the crystal structure of a class D beta-lactamase, the broad spectrum enzyme OXA-10 from Pseudomonas aeruginosa at 2.0 A resolution. There are significant differences between the overall fold observed in this structure and those of the evolutionarily related class A and class C beta-lactamases. Furthermore, the structure suggests the unique, cation mediated formation of a homodimer. Kinetic and hydrodynamic data shows that the dimer is a relevant species in solution and is the more active form of the enzyme. Comparison of the molecular details of the active sites of the class A and class C enzymes with the OXA-10 structure reveals that there is no counterpart in OXA-10 to the residues proposed to act as general bases in either of these enzymes (Glu 166 and Tyr 150, respectively). Our structures of the native and chloride inhibited forms of OXA-10 suggest that the class D enzymes have evolved a distinct catalytic mechanism for beta-lactam hydrolysis. Clinical variants of OXA-10 are also discussed in light of the structure.

About this Structure

1FOF is a Single protein structure of sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA.

Reference

Crystal structure of the class D beta-lactamase OXA-10., Paetzel M, Danel F, de Castro L, Mosimann SC, Page MG, Strynadka NC, Nat Struct Biol. 2000 Oct;7(10):918-25. PMID:11017203 Page seeded by OCA on Fri May 2 16:34:26 2008

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