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3fu1

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Revision as of 06:49, 1 November 2017

Crystal structure of the major pseudopilin from the type 2 secretion system of Vibrio cholerae

Structural highlights

3fu1 is a 2 chain structure with sequence from "bacillo_virgola_del_koch"_trevisan_1884 "bacillo virgola del koch" trevisan 1884. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Gene:	epsG, VC_2730 ("Bacillo virgola del Koch" Trevisan 1884)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[GSPG_VIBCH] Involved in a type II secretion system (T2SS, formerly general secretion pathway, GSP) for the export of proteins. Required for secretion of cholera toxin through the outer membrane.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The pseudopilus is a key feature of the type 2 secretion system (T2SS) and is made up of multiple pseudopilins that are similar in fold to the type 4 pilins. However, pilins have disulfide bridges, whereas the major pseudopilins of T2SS do not. A key question is therefore how the pseudopilins, and in particular, the most abundant major pseudopilin, GspG, obtain sufficient stability to perform their function. Crystal structures of Vibrio cholerae, Vibrio vulnificus, and enterohemorrhagic Escherichia coli (EHEC) GspG were elucidated, and all show a calcium ion bound at the same site. Conservation of the calcium ligands fully supports the suggestion that calcium ion binding by the major pseudopilin is essential for the T2SS. Functional studies of GspG with mutated calcium ion-coordinating ligands were performed to investigate this hypothesis and show that in vivo protease secretion by the T2SS is severely impaired. Taking all evidence together, this allows the conclusion that, in complete contrast to the situation in the type 4 pili system homologs, in the T2SS, the major protein component of the central pseudopilus is dependent on calcium ions for activity.

Calcium is essential for the major pseudopilin in the type 2 secretion system.,Korotkov KV, Gray MD, Kreger A, Turley S, Sandkvist M, Hol WG J Biol Chem. 2009 Sep 18;284(38):25466-70. Epub 2009 Jul 29. PMID:19640838^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Korotkov KV, Gray MD, Kreger A, Turley S, Sandkvist M, Hol WG. Calcium is essential for the major pseudopilin in the type 2 secretion system. J Biol Chem. 2009 Sep 18;284(38):25466-70. Epub 2009 Jul 29. PMID:19640838 doi:10.1074/jbc.C109.037655

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3fu1"

@@ Line 1: / Line 1: @@
 ==Crystal structure of the major pseudopilin from the type 2 secretion system of Vibrio cholerae==
 <StructureSection load='3fu1' size='340' side='right' caption='[[3fu1]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">epsG, VC_2730 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 "Bacillo virgola del Koch" Trevisan 1884])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fu1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fu1 OCA], [http://pdbe.org/3fu1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3fu1 RCSB], [http://www.ebi.ac.uk/pdbsum/3fu1 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fu1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fu1 OCA], [http://pdbe.org/3fu1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3fu1 RCSB], [http://www.ebi.ac.uk/pdbsum/3fu1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3fu1 ProSAT]</span></td></tr>
 </table>
 == Function ==

3fu1

From Proteopedia

Revision as of 06:49, 1 November 2017

Crystal structure of the major pseudopilin from the type 2 secretion system of Vibrio cholerae

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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