3dkn

From Proteopedia

(Difference between revisions)

Revision as of 07:55, 18 July 2018

Sec61 in the Canine ribosome-channel complex from the endoplasmic reticulum

Structural highlights

3dkn is a 6 chain structure with sequence from Canis lupus familiaris. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

During cotranslational protein translocation, the ribosome associates with a membrane channel, formed by the Sec61 complex, and recruits the translocon-associated protein complex (TRAP). Here we report the structure of a ribosome-channel complex from mammalian endoplasmic reticulum in which the channel has been visualized at 11 A resolution. In this complex, single copies of Sec61 and TRAP associate with a nontranslating ribosome and this stoichiometry was verified by quantitative mass spectrometry. A bilayer-like density surrounds the channel and can be attributed to lipid and detergent. The crystal structure of an archaeal homolog of the Sec61 complex was then docked into the map. In this model, two cytoplasmic loops of Sec61 may interact with RNA helices H6, H7, and H50, while the central pore is located below the ribosome tunnel exit. Hence, this copy of Sec61 is positioned to capture and translocate the nascent chain. Finally, we show that mammalian and bacterial ribosome-channel complexes have similar architectures.

Single copies of Sec61 and TRAP associate with a nontranslating mammalian ribosome.,Menetret JF, Hegde RS, Aguiar M, Gygi SP, Park E, Rapoport TA, Akey CW Structure. 2008 Jul;16(7):1126-37. PMID:18611385^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Menetret JF, Hegde RS, Aguiar M, Gygi SP, Park E, Rapoport TA, Akey CW. Single copies of Sec61 and TRAP associate with a nontranslating mammalian ribosome. Structure. 2008 Jul;16(7):1126-37. PMID:18611385 doi:10.1016/j.str.2008.05.003

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3dkn"

@@ Line 1: / Line 1: @@
 ==Sec61 in the Canine ribosome-channel complex from the endoplasmic reticulum==
 <StructureSection load='3dkn' size='340' side='right' caption='[[3dkn]], [[Resolution|resolution]] 8.70&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3dkn]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Canfa Canfa] and [http://en.wikipedia.org/wiki/Canis_lupus_familiaris Canis lupus familiaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DKN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3DKN FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3dkn]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Canis_lupus_familiaris Canis lupus familiaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DKN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3DKN FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3dkn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dkn OCA], [http://pdbe.org/3dkn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3dkn RCSB], [http://www.ebi.ac.uk/pdbsum/3dkn PDBsum]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3dkn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dkn OCA], [http://pdbe.org/3dkn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3dkn RCSB], [http://www.ebi.ac.uk/pdbsum/3dkn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3dkn ProSAT]</span></td></tr>
 </table>
 == Evolutionary Conservation ==
@@ Line 9: / Line 10: @@
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dk/3dkn_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dk/3dkn_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
@@ Line 31: / Line 32: @@
 __TOC__
 </StructureSection>
-[[Category: Canfa]]
 [[Category: Canis lupus familiaris]]
 [[Category: Akey, C]]

3dkn

From Proteopedia

Revision as of 07:55, 18 July 2018

Sec61 in the Canine ribosome-channel complex from the endoplasmic reticulum

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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