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3kxp

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Crystal Structure of E-2-(Acetamidomethylene)succinate Hydrolase

Structural highlights

3kxp is a 12 chain structure with sequence from Atcc 700743. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
NonStd Res:
Gene:	5331 (ATCC 700743)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The gene identification and kinetic characterization of (E)-2-(acetamidomethylene)succinate (E-2AMS) hydrolase has recently been described. This enzyme catalyzes the final reaction in the degradation of vitamin B(6) and produces succinic semialdehyde, acetate, ammonia, and carbon dioxide from E-2AMS. The structure of E-2AMS hydrolase was determined to 2.3 A using SAD phasing. E-2AMS hydrolase is a member of the alpha/beta hydrolase superfamily and utilizes a serine/histidine/aspartic acid catalytic triad. Mutation of either the nucleophilic serine or the aspartate resulted in inactive enzyme. Mutation of an additional serine residue in the active site causes the enzyme to be unstable and is likely structurally important. The structure also provides insight into the mechanism of hydrolysis of E-2AMS and identifies several potential catalytically important residues.

Structure Determination and Characterization of the Vitamin B(6) Degradative Enzyme (E)-2-(Acetamidomethylene)succinate Hydrolase (,).,McCulloch KM, Mukherjee T, Begley TP, Ealick SE Biochemistry. 2010 Feb 16;49(6):1226-35. PMID:20099871^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ McCulloch KM, Mukherjee T, Begley TP, Ealick SE. Structure Determination and Characterization of the Vitamin B(6) Degradative Enzyme (E)-2-(Acetamidomethylene)succinate Hydrolase (,). Biochemistry. 2010 Feb 16;49(6):1226-35. PMID:20099871 doi:10.1021/bi901812p

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3kxp"

@@ Line 1: / Line 1: @@
 ==Crystal Structure of E-2-(Acetamidomethylene)succinate Hydrolase==
 <StructureSection load='3kxp' size='340' side='right' caption='[[3kxp]], [[Resolution|resolution]] 2.26&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3kxp]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_33669 Atcc 33669]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KXP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3KXP FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3kxp]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_700743 Atcc 700743]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KXP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3KXP FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
 <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">5331 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=381 ATCC 33669])</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">5331 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=381 ATCC 700743])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3kxp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kxp OCA], [http://pdbe.org/3kxp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3kxp RCSB], [http://www.ebi.ac.uk/pdbsum/3kxp PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3kxp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kxp OCA], [http://pdbe.org/3kxp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3kxp RCSB], [http://www.ebi.ac.uk/pdbsum/3kxp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3kxp ProSAT]</span></td></tr>
 </table>
 == Evolutionary Conservation ==
@@ Line 31: / Line 32: @@
 __TOC__
 </StructureSection>
-[[Category: Atcc 33669]]
+[[Category: Atcc 700743]]
 [[Category: Begley, T P]]
 [[Category: Ealick, S E]]

3kxp

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Current revision

Crystal Structure of E-2-(Acetamidomethylene)succinate Hydrolase

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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