1fvi

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[[Image:1fvi.gif|left|200px]]
[[Image:1fvi.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1fvi |SIZE=350|CAPTION= <scene name='initialview01'>1fvi</scene>, resolution 2.0&Aring;
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The line below this paragraph, containing "STRUCTURE_1fvi", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1fvi| PDB=1fvi | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fvi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fvi OCA], [http://www.ebi.ac.uk/pdbsum/1fvi PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1fvi RCSB]</span>
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}}
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'''CRYSTAL STRUCTURE OF CHLORELLA VIRUS DNA LIGASE-ADENYLATE'''
'''CRYSTAL STRUCTURE OF CHLORELLA VIRUS DNA LIGASE-ADENYLATE'''
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[[Category: Shuman, S.]]
[[Category: Shuman, S.]]
[[Category: Sriskanda, V.]]
[[Category: Sriskanda, V.]]
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[[Category: adenylated dna ligase]]
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[[Category: Adenylated dna ligase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 16:48:36 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:31:03 2008''
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Revision as of 13:48, 2 May 2008

Image:1fvi.gif

Template:STRUCTURE 1fvi

CRYSTAL STRUCTURE OF CHLORELLA VIRUS DNA LIGASE-ADENYLATE


Overview

Chlorella virus DNA ligase is the smallest eukaryotic ATP-dependent ligase known; it has an intrinsic nick-sensing function and suffices for yeast cell growth. Here, we report the 2.0 A crystal structure of the covalent ligase-AMP reaction intermediate. The conformation of the adenosine nucleoside and contacts between the enzyme and the ribose sugar have undergone a significant change compared to complexes of T7 ligase with ATP or mRNA capping enzyme with GTP. The conformational switch allows the 3' OH of AMP to coordinate directly the 5' PO(4) of the nick. The structure explains why nick sensing is restricted to adenylated ligase and why the 5' phosphate is required for DNA binding. We identify a metal binding site on ligase-adenylate and propose a mechanism of nick recognition and catalysis supported by mutational data.

About this Structure

1FVI is a Single protein structure of sequence from Chlorella virus. The following page contains interesting information on the relation of 1FVI with [DNA Ligase]. Full crystallographic information is available from OCA.

Reference

Crystal structure of eukaryotic DNA ligase-adenylate illuminates the mechanism of nick sensing and strand joining., Odell M, Sriskanda V, Shuman S, Nikolov DB, Mol Cell. 2000 Nov;6(5):1183-93. PMID:11106756 Page seeded by OCA on Fri May 2 16:48:36 2008

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