1fyo
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1fyo.gif|left|200px]] | [[Image:1fyo.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1fyo", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | | | + | or leave the SCENE parameter empty for the default display. |
| - | | | + | --> |
| - | + | {{STRUCTURE_1fyo| PDB=1fyo | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''EUKARYOTIC DECODING REGION A-SITE RNA''' | '''EUKARYOTIC DECODING REGION A-SITE RNA''' | ||
| Line 19: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FYO OCA]. | |
==Reference== | ==Reference== | ||
Structure of a eukaryotic decoding region A-site RNA., Lynch SR, Puglisi JD, J Mol Biol. 2001 Mar 9;306(5):1023-35. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11237616 11237616] | Structure of a eukaryotic decoding region A-site RNA., Lynch SR, Puglisi JD, J Mol Biol. 2001 Mar 9;306(5):1023-35. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11237616 11237616] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Lynch, S R.]] | [[Category: Lynch, S R.]] | ||
[[Category: Puglisi, J D.]] | [[Category: Puglisi, J D.]] | ||
| - | [[Category: | + | [[Category: Bulged some]] |
| - | [[Category: | + | [[Category: G-a base pair]] |
| - | [[Category: | + | [[Category: Stem-internal loop-stem-tetraloop rna]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 16:54:56 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 13:54, 2 May 2008
EUKARYOTIC DECODING REGION A-SITE RNA
Overview
The aminoglycoside antibiotics target a region of highly conserved nucleotides in the aminoacyl-tRNA site (A site) of 16 S RNA on the 30 S subunit. The structures of a prokaryotic decoding region A-site oligonucleotide free in solution and bound to the aminoglycosides paromomycin and gentamicin C1A have been determined. Here, the structure of a eukaryotic decoding region A-site oligonucleotide has been determined using homonuclear and heteronuclear NMR spectroscopy, and compared to the unbound prokaryotic rRNA structure. The two structures are similar, with a U1406-U1495 base-pair, a C1407-G1494 Watson-Crick base-pair, and a G1408-A1493 base-pair instead of the A1408-A1493 base-pair of the prokaryotic structure. The two structures differ in the orientation of the 1408 position with respect to A1493; G1408 is rotated toward the major groove, which is the binding pocket for aminoglycosides. The structures also differ in the stacking geometry of G1494 on A1493, which could have slight long-range conformational effects.
About this Structure
Full crystallographic information is available from OCA.
Reference
Structure of a eukaryotic decoding region A-site RNA., Lynch SR, Puglisi JD, J Mol Biol. 2001 Mar 9;306(5):1023-35. PMID:11237616 Page seeded by OCA on Fri May 2 16:54:56 2008
