Luciferase
From Proteopedia
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<StructureSection load='3iep' size='350' side='right' scene='Luciferase/Cv/1' caption='Luciferase (PDB code [[3iep]])'> | <StructureSection load='3iep' size='350' side='right' scene='Luciferase/Cv/1' caption='Luciferase (PDB code [[3iep]])'> | ||
| - | + | == Function == | |
| - | [[Luciferase]] (Luc) is an oxidative enzyme used in bioluminescence. It catalyzes the oxidation of the pigment luciferin producing oxyluciferin and light. See detailed analysis in<br /> | + | [[Luciferase]] (Luc) is an oxidative enzyme used in bioluminescence. It catalyzes the oxidation of the pigment luciferin producing oxyluciferin and light<ref>PMID:2030669</ref>. See detailed analysis in<br /> |
* [[Luciola cruciata luciferase]]<br /> | * [[Luciola cruciata luciferase]]<br /> | ||
* [[Luciferase FMN complex- Vibrio harveyi]]<br /> | * [[Luciferase FMN complex- Vibrio harveyi]]<br /> | ||
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See [[Colored & Bioluminescent Protein]]. | See [[Colored & Bioluminescent Protein]]. | ||
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| + | == Relevance == | ||
| + | Luc is used in biological research to assess gene transfection into cells where the Luc gene is added to the transfected gene and the light emission by Luc measured for indication of the transfection success<ref>PMID:3072883</ref>. | ||
== 3D Structures of Luciferase == | == 3D Structures of Luciferase == | ||
Revision as of 08:56, 13 April 2016
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References
- ↑ Meighen EA. Molecular biology of bacterial bioluminescence. Microbiol Rev. 1991 Mar;55(1):123-42. PMID:2030669
- ↑ Gould SJ, Subramani S. Firefly luciferase as a tool in molecular and cell biology. Anal Biochem. 1988 Nov 15;175(1):5-13. PMID:3072883
- ↑ Auld DS, Lovell S, Thorne N, Lea WA, Maloney DJ, Shen M, Rai G, Battaile KP, Thomas CJ, Simeonov A, Hanzlik RP, Inglese J. Molecular basis for the high-affinity binding and stabilization of firefly luciferase by PTC124. Proc Natl Acad Sci U S A. 2010 Mar 16;107(11):4878-83. Epub 2010 Mar 1. PMID:20194791
- ↑ Campbell ZT, Weichsel A, Montfort WR, Baldwin TO. Crystal Structure of the Bacterial Luciferase:Flavin Complex Provides Insight into the Function of the subunit. Biochemistry. 2009 May 12. PMID:19435287 doi:http://dx.doi.org/10.1021/bi900003t
Additional Literature
- Marques SM, Esteves da Silva JC. Firefly bioluminescence: a mechanistic approach of luciferase catalyzed reactions. IUBMB Life. 2009 Jan;61(1):6-17. PMID:18949818 doi:10.1002/iub.134
- Inouye S. Firefly luciferase: an adenylate-forming enzyme for multicatalytic functions. Cell Mol Life Sci. 2010 Feb;67(3):387-404. Epub 2009 Oct 27. PMID:19859663 doi:10.1007/s00018-009-0170-8
External Resources
- Yahoo news coverage of luciferase studies funded research for military applications
- Luciferase at Wikipedia
- Luciferase: June 2006 Molecule of the Month as part of the series of tutorials that are at the RCSB Protein Data Bank and written by David S. Goodsell
Proteopedia Page Contributors and Editors (what is this?)
Michal Harel, Wayne Decatur, Alexander Berchansky, Loïc Gazquez, Charlene Planchenault, David Canner
