1g60
From Proteopedia
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'''Crystal Structure of Methyltransferase MboIIa (Moraxella bovis)''' | '''Crystal Structure of Methyltransferase MboIIa (Moraxella bovis)''' | ||
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[[Category: Moraxella bovis]] | [[Category: Moraxella bovis]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Site-specific DNA-methyltransferase (adenine-specific)]] | ||
[[Category: Joachimiak, A.]] | [[Category: Joachimiak, A.]] | ||
[[Category: MCSG, Midwest Center for Structural Genomics.]] | [[Category: MCSG, Midwest Center for Structural Genomics.]] | ||
[[Category: Osipiuk, J.]] | [[Category: Osipiuk, J.]] | ||
[[Category: Walsh, M A.]] | [[Category: Walsh, M A.]] | ||
| - | [[Category: | + | [[Category: Dna methylation]] |
| - | [[Category: | + | [[Category: Mcsg]] |
| - | [[Category: | + | [[Category: Midwest center for structural genomic]] |
| - | [[Category: | + | [[Category: Moraxella bovi]] |
| - | [[Category: | + | [[Category: Protein structure initiative]] |
| - | [[Category: | + | [[Category: Psi]] |
| - | [[Category: | + | [[Category: S-adenosylmethionine]] |
| - | [[Category: | + | [[Category: Structural genomic]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:10:51 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 14:10, 2 May 2008
Crystal Structure of Methyltransferase MboIIa (Moraxella bovis)
Overview
DNA methyltransferases (MTases) are sequence-specific enzymes which transfer a methyl group from S-adenosyl-L-methionine (AdoMet) to the amino group of either cytosine or adenine within a recognized DNA sequence. Methylation of a base in a specific DNA sequence protects DNA from nucleolytic cleavage by restriction enzymes recognizing the same DNA sequence. We have determined at 1.74 A resolution the crystal structure of a beta-class DNA MTase MboIIA (M.MboIIA) from the bacterium Moraxella bovis, the smallest DNA MTase determined to date. M.MboIIA methylates the 3' adenine of the pentanucleotide sequence 5'-GAAGA-3'. The protein crystallizes with two molecules in the asymmetric unit which we propose to resemble the dimer when M.MboIIA is not bound to DNA. The overall structure of the enzyme closely resembles that of M.RsrI. However, the cofactor-binding pocket in M.MboIIA forms a closed structure which is in contrast to the open-form structures of other known MTases.
About this Structure
1G60 is a Single protein structure of sequence from Moraxella bovis. Full crystallographic information is available from OCA.
Reference
Crystal structure of MboIIA methyltransferase., Osipiuk J, Walsh MA, Joachimiak A, Nucleic Acids Res. 2003 Sep 15;31(18):5440-8. PMID:12954781 Page seeded by OCA on Fri May 2 17:10:51 2008
Categories: Moraxella bovis | Single protein | Joachimiak, A. | MCSG, Midwest Center for Structural Genomics. | Osipiuk, J. | Walsh, M A. | Dna methylation | Mcsg | Midwest center for structural genomic | Moraxella bovi | Protein structure initiative | Psi | S-adenosylmethionine | Structural genomic
