1e72
From Proteopedia
(New page: 200px<br /> <applet load="1e72" size="450" color="white" frame="true" align="right" spinBox="true" caption="1e72, resolution 1.6Å" /> '''MYROSINASE FROM SINA...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1E72 is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/ ]] with NAG, ZN, SO4, ASC, GOX and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.3.1 3.2.3.1]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1E72 OCA]]. | + | 1E72 is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Sinapis_alba Sinapis alba]] with NAG, ZN, SO4, ASC, GOX and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Transferred_entry:_3.2.1.147 Transferred entry: 3.2.1.147]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.3.1 3.2.3.1]]. Structure known Active Sites: ACT, ASC and ZNB. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1E72 OCA]]. |
==Reference== | ==Reference== | ||
High resolution X-ray crystallography shows that ascorbate is a cofactor for myrosinase and substitutes for the function of the catalytic base., Burmeister WP, Cottaz S, Rollin P, Vasella A, Henrissat B, J Biol Chem. 2000 Dec 15;275(50):39385-93. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10978344 10978344] | High resolution X-ray crystallography shows that ascorbate is a cofactor for myrosinase and substitutes for the function of the catalytic base., Burmeister WP, Cottaz S, Rollin P, Vasella A, Henrissat B, J Biol Chem. 2000 Dec 15;275(50):39385-93. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10978344 10978344] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| + | [[Category: Sinapis alba]] | ||
| + | [[Category: Transferred entry: 3.2.1.147]] | ||
[[Category: Burmeister, W.P.]] | [[Category: Burmeister, W.P.]] | ||
[[Category: ASC]] | [[Category: ASC]] | ||
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[[Category: transition state analogue]] | [[Category: transition state analogue]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:03:36 2007'' |
Revision as of 08:58, 30 October 2007
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MYROSINASE FROM SINAPIS ALBA WITH BOUND GLUCO-HYDROXIMOLACTAM AND SULFATE OR ASCORBATE
Overview
Myrosinase, an S-glycosidase, hydrolyzes plant anionic, 1-thio-beta-d-glucosides (glucosinolates) considered part of the plant, defense system. Although O-glycosidases are ubiquitous, myrosinase is the, only known S-glycosidase. Its active site is very similar to that of, retaining O-glycosidases, but one of the catalytic residues in, O-glycosidases, a carboxylate residue functioning as the general base, is, replaced by a glutamine residue. Myrosinase is strongly activated by, ascorbic acid. Several binary and ternary complexes of myrosinase with, different transition state analogues and ascorbic acid have been analyzed, at high resolution by x-ray crystallography along with a, 2-deoxy-2-fluoro-glucosyl enzyme intermediate. One of the inhibitors, d-gluconhydroximo-1,5-lactam, binds ... [(full description)]
About this Structure
1E72 is a [Protein complex] structure of sequences from [Sinapis alba] with NAG, ZN, SO4, ASC, GOX and GOL as [ligands]. Active as [Transferred entry: 3.2.1.147], with EC number [3.2.3.1]. Structure known Active Sites: ACT, ASC and ZNB. Full crystallographic information is available from [OCA].
Reference
High resolution X-ray crystallography shows that ascorbate is a cofactor for myrosinase and substitutes for the function of the catalytic base., Burmeister WP, Cottaz S, Rollin P, Vasella A, Henrissat B, J Biol Chem. 2000 Dec 15;275(50):39385-93. PMID:10978344
Page seeded by OCA on Tue Oct 30 11:03:36 2007
Categories: Protein complex | Sinapis alba | Transferred entry: 3.2.1.147 | Burmeister, W.P. | ASC | GOL | GOX | NAG | SO4 | ZN | Activation | Ascorbate | Family 1 glycosyl hydrolase | Glucosinolate | Inhibitor | Myrosinase | Tim barrel | Transition state analogue
