1g92
From Proteopedia
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[[Image:1g92.jpg|left|200px]] | [[Image:1g92.jpg|left|200px]] | ||
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| - | + | {{STRUCTURE_1g92| PDB=1g92 | SCENE= }} | |
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'''SOLUTION STRUCTURE OF PONERATOXIN''' | '''SOLUTION STRUCTURE OF PONERATOXIN''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1G92 is a [[Single protein]] structure | + | 1G92 is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G92 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Poznanski, J.]] | [[Category: Poznanski, J.]] | ||
[[Category: Szolajska, E.]] | [[Category: Szolajska, E.]] | ||
| - | [[Category: | + | [[Category: Neurotoxin]] |
| - | [[Category: | + | [[Category: Sodium channel inhibitor]] |
| - | [[Category: | + | [[Category: Toxin]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:17:54 2008'' | |
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Revision as of 14:17, 2 May 2008
SOLUTION STRUCTURE OF PONERATOXIN
Overview
Poneratoxin is a small neuropeptide found in the venom of the ant Paraponera clavata. It is stored in the venom reservoir as an inactive 25-residue peptide. Here we describe both chemically synthesized poneratoxin and poneratoxin obtained by expression in insect cells. When expressed in insect cells, poneratoxin was observed attached to cell membranes. Both synthetic and recombinant ponerotoxins were soluble below pH 4.5. The structure of synthetic poneratoxin was characterized by circular dichroism and solved by nuclear magnetic resonance. In an environment imitating a lipid bilayer, at pH within the range of insect hemolymph, synthetic poneratoxin has a V shape, with two alpha-helices connected by a beta-turn. Insect larvae were paralyzed by injection of either of the purified toxins, with the recombinant one acting faster. The recombinant toxin-producing baculovirus reduced the average survival time of the insect host by 25 h compared with unmodified virus. Mass spectrometry analysis showed that the recombinant toxin has an N-terminal 21-residue extension, possibly improving its stability and/or stabilizing the membrane-bound state. The potential use of poneratoxin for the construction of biological insecticide is discussed.
About this Structure
1G92 is a Single protein structure. Full crystallographic information is available from OCA.
Reference
Poneratoxin, a neurotoxin from ant venom. Structure and expression in insect cells and construction of a bio-insecticide., Szolajska E, Poznanski J, Ferber ML, Michalik J, Gout E, Fender P, Bailly I, Dublet B, Chroboczek J, Eur J Biochem. 2004 Jun;271(11):2127-36. PMID:15153103 Page seeded by OCA on Fri May 2 17:17:54 2008
