5hoe
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structrue of Est24, a carbohydrate acetylesterase from Sinorhizobium meliloti== | |
| + | <StructureSection load='5hoe' size='340' side='right' caption='[[5hoe]], [[Resolution|resolution]] 1.45Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5hoe]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HOE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HOE FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5hoe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hoe OCA], [http://pdbe.org/5hoe PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hoe RCSB], [http://www.ebi.ac.uk/pdbsum/5hoe PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Carbohydrate acetylesterases, which have a highly specific role among plant-interacting bacterial species, remove the acetyl groups from plant carbohydrates. Here, we determined the crystal structure of Est24, an octameric carbohydrate acetylesterase from Sinorhizobium meliloti, at 1.45 A resolution and investigated its biochemical properties. The structure of Est24 consisted of five parallel beta strands flanked by alpha helices, which formed an octameric assembly with two distinct interfaces. The deacetylation activity of Est24 and its mutants around the substrate-binding pocket was investigated using several substrates, including glucose pentaacetate and acetyl alginate. Elucidation of the structure-function relationships of Est24 could provide valuable opportunities for biotechnological explorations. | ||
| - | + | Structural and Biochemical Characterization of an Octameric Carbohydrate Acetylesterase from Sinorhizobium meliloti.,Oh C, Ryu BH, An DR, Nguyen DD, Yoo W, Kim T, Ngo TD, Kim HS, Kim KK, Kim TD FEBS Lett. 2016 Apr;590(8):1242-52. doi: 10.1002/1873-3468.12135. Epub 2016 Mar, 31. PMID:26991446<ref>PMID:26991446</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 5hoe" style="background-color:#fffaf0;"></div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | + | __TOC__ | |
| - | [[Category: An, D | + | </StructureSection> |
| - | [[Category: | + | [[Category: An, D R]] |
| - | [[Category: Kim, | + | [[Category: Kim, H S]] |
| - | + | [[Category: Kim, K K]] | |
[[Category: Kim, T]] | [[Category: Kim, T]] | ||
| - | [[Category: Kim, | + | [[Category: Kim, T D]] |
| - | [[Category: | + | [[Category: Ngo, D T]] |
| + | [[Category: Nguyen, D D]] | ||
| + | [[Category: Oh, C]] | ||
| + | [[Category: Park, J S]] | ||
| + | [[Category: Ryu, B H]] | ||
| + | [[Category: Yoo, W]] | ||
| + | [[Category: Carbohydrate acetylesterase]] | ||
| + | [[Category: Est24]] | ||
| + | [[Category: Hydrolase]] | ||
| + | [[Category: Sinorhizobium meliloti]] | ||
Revision as of 12:06, 13 May 2016
Crystal structrue of Est24, a carbohydrate acetylesterase from Sinorhizobium meliloti
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Categories: An, D R | Kim, H S | Kim, K K | Kim, T | Kim, T D | Ngo, D T | Nguyen, D D | Oh, C | Park, J S | Ryu, B H | Yoo, W | Carbohydrate acetylesterase | Est24 | Hydrolase | Sinorhizobium meliloti
