1gcc

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[[Image:1gcc.gif|left|200px]]
[[Image:1gcc.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1gcc |SIZE=350|CAPTION= <scene name='initialview01'>1gcc</scene>
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The line below this paragraph, containing "STRUCTURE_1gcc", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE= ATERF1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana])
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-->
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|DOMAIN=
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{{STRUCTURE_1gcc| PDB=1gcc | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gcc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gcc OCA], [http://www.ebi.ac.uk/pdbsum/1gcc PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1gcc RCSB]</span>
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}}
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'''SOLUTION NMR STRUCTURE OF THE COMPLEX OF GCC-BOX BINDING DOMAIN OF ATERF1 AND GCC-BOX DNA, MINIMIZED AVERAGE STRUCTURE'''
'''SOLUTION NMR STRUCTURE OF THE COMPLEX OF GCC-BOX BINDING DOMAIN OF ATERF1 AND GCC-BOX DNA, MINIMIZED AVERAGE STRUCTURE'''
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[[Category: Tateno, M.]]
[[Category: Tateno, M.]]
[[Category: Yamasaki, K.]]
[[Category: Yamasaki, K.]]
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[[Category: complex (transcription factor/dna)]]
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[[Category: Ethylene inducible]]
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[[Category: ethylene inducible]]
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[[Category: Protein-dna complex]]
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[[Category: protein-dna complex]]
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[[Category: Transcription factor]]
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[[Category: transcription factor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:24:35 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:41:17 2008''
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Revision as of 14:24, 2 May 2008

Image:1gcc.gif

Template:STRUCTURE 1gcc

SOLUTION NMR STRUCTURE OF THE COMPLEX OF GCC-BOX BINDING DOMAIN OF ATERF1 AND GCC-BOX DNA, MINIMIZED AVERAGE STRUCTURE


Overview

The 3D solution structure of the GCC-box binding domain of a protein from Arabidopsis thaliana in complex with its target DNA fragment has been determined by heteronuclear multidimensional NMR in combination with simulated annealing and restrained molecular dynamic calculation. The domain consists of a three-stranded anti-parallel beta-sheet and an alpha-helix packed approximately parallel to the beta-sheet. Arginine and tryptophan residues in the beta-sheet are identified to contact eight of the nine consecutive base pairs in the major groove, and at the same time bind to the sugar phosphate backbones. The target DNA bends slightly at the central CG step, thereby allowing the DNA to follow the curvature of the beta-sheet.

About this Structure

1GCC is a Single protein structure of sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA.

Reference

A novel mode of DNA recognition by a beta-sheet revealed by the solution structure of the GCC-box binding domain in complex with DNA., Allen MD, Yamasaki K, Ohme-Takagi M, Tateno M, Suzuki M, EMBO J. 1998 Sep 15;17(18):5484-96. PMID:9736626 Page seeded by OCA on Fri May 2 17:24:35 2008

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