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5i7z
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of a Par-6 PDZ-Crumbs 3 C-terminal peptide complex== | |
| + | <StructureSection load='5i7z' size='340' side='right' caption='[[5i7z]], [[Resolution|resolution]] 1.80Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5i7z]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I7Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5I7Z FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5i7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i7z OCA], [http://pdbe.org/5i7z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5i7z RCSB], [http://www.ebi.ac.uk/pdbsum/5i7z PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Par-6 is a scaffold protein that organizes other proteins into a complex required to initiate and maintain cell polarity. Cdc42-GTP binds the CRIB module of Par-6 and alters the binding affinity of the adjoining PDZ domain. Allosteric regulation of the Par-6 PDZ domain was first demonstrated using a peptide identified in a screen of typical carboxyl-terminal ligands. Crumbs, a membrane protein that localizes a conserved polarity complex, was subsequently identified as a functional partner for Par-6 that likely interacts with the PDZ domain. Here we show by nuclear magnetic resonance that Par-6 binds a Crumbs carboxyl-terminal peptide and report the crystal structure of the PDZ-peptide complex. The Crumbs peptide binds Par-6 more tightly than the previously studied carboxyl peptide ligand and interacts with the CRIB-PDZ module in a Cdc42-dependent manner. The Crumbs:Par-6 crystal structure reveals specific PDZ-peptide contacts that contribute to its higher affinity and Cdc42-enhanced binding. Comparisons with existing structures suggest that multiple C-terminal Par-6 ligands respond to a common conformational switch that transmits the allosteric effects of GTPase binding. | ||
| - | + | Binding of Crumbs to the Par-6 CRIB-PDZ Module Is Regulated by Cdc42.,Whitney DS, Peterson FC, Kittell AW, Egner JM, Prehoda KE, Volkman BF Biochemistry. 2016 Mar 15;55(10):1455-61. doi: 10.1021/acs.biochem.5b01342. Epub , 2016 Mar 3. PMID:26894406<ref>PMID:26894406</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Prehoda, K | + | <div class="pdbe-citations 5i7z" style="background-color:#fffaf0;"></div> |
| - | [[Category: Whitney, D | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: | + | __TOC__ |
| + | </StructureSection> | ||
| + | [[Category: Peterson, F C]] | ||
| + | [[Category: Prehoda, K E]] | ||
| + | [[Category: Volkman, B F]] | ||
| + | [[Category: Whitney, D S]] | ||
| + | [[Category: Cell polarity]] | ||
| + | [[Category: Pdz]] | ||
| + | [[Category: Signaling protein]] | ||
Revision as of 18:13, 10 May 2016
Crystal structure of a Par-6 PDZ-Crumbs 3 C-terminal peptide complex
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