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Sandbox Wabash 27 Fumarase
From Proteopedia
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==Fumarase: The Active Site Debate Answered== | ==Fumarase: The Active Site Debate Answered== | ||
<StructureSection load='1YFE' size='340' side='right' caption='Fumarase' scene=''> | <StructureSection load='1YFE' size='340' side='right' caption='Fumarase' scene=''> | ||
| - | Fumarase (fumarate hydratase) is an enzyme found in eukaryotic cells which catalyzes the reaction between L-malate and fumarate.<ref>PMID: 9098893</ref> The catalysis proceeds via the deprotination of the C3 carbon of L-malate, which is followed by the loss of the -OH group attached to the C2 carbon; the intermediate for which is a carbanion transition state.<ref>Voet, Donald, Judith G. Voet, and Charlotte W. Pratt. Fundamentals of Biochemistry. 3rd ed. New York: Wiley, 1999. Print.</ref> | + | Fumarase C (fumarate hydratase) is an enzyme found in eukaryotic cells which catalyzes the reaction between L-malate and fumarate.<ref>PMID: 9098893</ref> The catalysis proceeds via the deprotination of the C3 carbon of L-malate, which is followed by the loss of the -OH group attached to the C2 carbon; the intermediate for which is a carbanion transition state.<ref>Voet, Donald, Judith G. Voet, and Charlotte W. Pratt. Fundamentals of Biochemistry. 3rd ed. New York: Wiley, 1999. Print.</ref> In order for this catalysis to occur L-malate must be bound to fumarase. Studies have determined that fumarase has two carboxylic binding sites (site-A and site-B) which could potentially be the active site for the production of fumarate. However, it has been experimentally determined that binding site-A is in fact the active site of fumarase for the process of converting L-malate to fumarate. |
| - | == | + | == Binding Site Characteristics == |
| - | + | Site-A: Site-A can be described as being comprised of atoms from three of the four sub-units (tetramer). | |
| - | + | Site-B: | |
| - | == H188N == | + | == H188N: Effects of Mutation == |
H188N is used to refer to the fumarase C with five histidines on the C-terminal with histidine 188 mutated to asparagine. | H188N is used to refer to the fumarase C with five histidines on the C-terminal with histidine 188 mutated to asparagine. | ||
| - | == H129N == | + | == H129N: Effects of Mutation == |
H129N is used to refer to the fumarase C with five histidines on the C-terminal with histidine 129 mutated to asparagine. | H129N is used to refer to the fumarase C with five histidines on the C-terminal with histidine 129 mutated to asparagine. | ||
Revision as of 03:16, 28 February 2016
Fumarase: The Active Site Debate Answered
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References
- ↑ Weaver T, Lees M, Banaszak L. Mutations of fumarase that distinguish between the active site and a nearby dicarboxylic acid binding site. Protein Sci. 1997 Apr;6(4):834-42. PMID:9098893
- ↑ Voet, Donald, Judith G. Voet, and Charlotte W. Pratt. Fundamentals of Biochemistry. 3rd ed. New York: Wiley, 1999. Print.
